Analysis of the role of RsbV, RsbW, and RsbY in regulating {sigma}B activity in Bacillus cereus.

Wageningen Centre for Food Sciences, Wageningen, The Netherlands.
Journal of Bacteriology (Impact Factor: 2.69). 09/2005; 187(16):5846-51. DOI: 10.1128/JB.187.16.5846-5851.2005
Source: PubMed

ABSTRACT The alternative sigma factor sigma(B) is an important regulator of the stress response of Bacillus cereus. Here, the role of the regulatory proteins RsbV, RsbW, and RsbY in regulating sigma(B) activity in B. cereus is analyzed. Functional characterization of RsbV and RsbW showed that they act as an anti-sigma factor antagonist and an anti-sigma factor, respectively. RsbW can also act as a kinase on RsbV. These data are in line with earlier functional characterizations of RsbV and RsbW homologs in B. subtilis. The rsbY gene is unique to B. cereus and its closest relatives and is predicted to encode a protein with an N-terminal CheY domain and a C-terminal PP2C domain. In an rsbY deletion mutant, the sigma(B) response upon stress exposure was almost completely abolished, but the response could be restored by complementation with full-length rsbY. Expression analysis showed that rsbY is transcribed from both a sigma(A)-dependent promoter and a sigma(B)-dependent promoter. The central role of RsbY in regulating the activity of sigma(B) indicates that in B. cereus, the sigma(B) activation pathway is markedly different from that in other gram-positive bacteria.

Download full-text


Available from: Marcel Zwietering, Jul 29, 2015
  • Source
    • "These include Bacillus cereus (van Schaik et al., 2005 "
    [Show abstract] [Hide abstract]
    ABSTRACT: Bacteria employ a variety of mechanisms to promote and control colonization of their respective hosts, including restricting the expression of genes necessary for colonization to distinct situations (i.e. encounter with a prospective host). In the symbiosis between the marine bacterium Vibrio fischeri and its host squid, Euprymna scolopes, colonization proceeds via a transient biofilm formed by the bacterium. The production of this bacterial biofilm depends on a complex regulatory network that controls transcription of the symbiosis polysaccharide (syp) gene locus. In addition to this transcriptional control, biofilm formation is regulated by two proteins, SypA and SypE, which may function in an unusual regulatory mechanism known as partner switching. Best characterized in Bacillus subtilis and other Gram-positive bacteria, partner switching is a signalling mechanism that provides dynamic regulatory control over bacterial gene expression. The involvement of putative partner-switching components within V. fischeri suggests that tight regulatory control over biofilm formation may be important for the lifestyle of this organism.
    Environmental Microbiology 08/2010; 12(8):2051-9. DOI:10.1111/j.1462-2920.2010.02269.x · 6.24 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: This paper reports on the development of an ultrahigh accuracy distance meter (femtosecond-comb distance meter (FCDM)) with a simple setup and reliability for a practical measurement using a broad and stable femtosecond frequency comb. The distance meter is expected for a length standard as well as industrial applications. In this study, a 2-μm uncertainty in the FCDM by using 10-GHz beat frequencies is achieved.
    Holey Fibers and Photonic Crystals/Polarization Mode Dispersion/Photonics Time/Frequency Measurement and Control, 2003 Digest of the LEOS Summer Topical Meetings; 08/2003
  • Source
    Journal of Bacteriology 06/2006; 188(10):3433-41. DOI:10.1128/JB.188.10.3433-3441.2006 · 2.69 Impact Factor
Show more