Article

Phenotypic and genotypic properties of Streptococcus equi subsp. zooepidemicus isolated from harbor seals (Phoca vitulina) from the German North Sea during the phocine distemper outbreak in 2002.

Institut für Tierärztliche Nahrungsmittelkunde, Justus-Liebig-Universität Giessen, Giessen, Germany.
Veterinary Microbiology (impact factor: 3.33). 10/2005; 110(1-2):147-52. DOI:10.1016/j.vetmic.2005.06.010 pp.147-52
Source: PubMed

ABSTRACT The present study was designed to identify and compare 32 beta-hemolytic streptococci isolated from 28 different harbor seals of the German North Sea during the phocine distemper outbreak in 2002. The bacteria were identified as Streptococcus equi subsp. zooepidemicus based on cultural, biochemical, serological and molecular studies. Epidemiological investigations by PCR restriction fragment length polymorphism analysis of the 16S-23S rDNA intergenic spacer region and gene szp and by macrorestriction analysis of the chromosomal DNA of the strains by pulsed field gel electrophoresis revealed that all 32 strains appeared to be identical. These results indicate that a single bacterial clone seemed to be distributed among the harbor seal population of the German North Sea during this outbreak.

0 0
 · 
0 Bookmarks
 · 
36 Views
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

16S-23S rDNA intergenic spacer region
 
28 different harbor
 
biochemical
 
German North Sea
 
identical
 
macrorestriction analysis
 
PCR restriction fragment length polymorphism analysis
 
phocine distemper outbreak
 
pulsed field gel electrophoresis
 
single bacterial clone
 
Streptococcus equi subsp