Article

Separation and determination of clotrimazole, methylparaben and propylparaben in pharmaceutical preparation by micellar electrokinetic chromatography.

Charles University, Faculty of Pharmacy, Department of Analytical Chemistry, Heyrovského 1203, CZ-500 05 Hradec Králové, Czech Republic.
Journal of Pharmaceutical and Biomedical Analysis (impact factor: 2.97). 01/2006; 40(1):215-9. DOI:10.1016/j.jpba.2005.07.001
Source: PubMed

ABSTRACT In this study, micellar electrokinetic chromatography (MEKC) method was developed for the determination of clotrimazole (CLO), methylparaben (MP) and propylparaben (PP) in a pharmaceutical preparation. Separation was carried out in a fused silica capillary (60 cm x 75 microm i.d.) at 25 kV with UV detection at 212 nm. Optimized background electrolyte (BGE) was 15 mM phosphate buffer (pH 7.2) containing 30 mM sodium dodecyl sulfate (SDS) as a surfactant. Rectilinear calibration ranges were 50-500 mg l(-1) for CLO, 10-100 mg l(-1) for MP and 2.5-25 mg l(-1) for PP. The total analysis time was < 12 min.

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Keywords

clotrimazole
 
fused silica capillary
 
micellar electrokinetic chromatography
 
Optimized background electrolyte
 
pharmaceutical preparation
 
surfactant
 
total analysis time
 
UV detection