Rau V, DeCola JP, Fanselow MS. Stress-induced enhancement of fear learning: an animal model of posttraumatic stress disorder. Neurosci Biobehav Rev 29: 1207-1223

Department of Psychology, University of California, Los Angeles, 415 Hilgard Ave, Los Angeles, CA 90095-1563, USA.
Neuroscience & Biobehavioral Reviews (Impact Factor: 8.8). 02/2005; 29(8):1207-23. DOI: 10.1016/j.neubiorev.2005.04.010
Source: PubMed


Fear is an adaptive response that initiates defensive behavior to protect animals and humans from danger. However, anxiety disorders, such as Posttraumatic Stress Disorder (PTSD), can occur when fear is inappropriately regulated. Fear conditioning can be used to study aspects of PTSD, and we have developed a model in which pre-exposure to a stressor of repeated footshock enhances conditional fear responding to a single context-shock pairing. The experiments in this chapter address interpretations of this effect including generalization and summation or fear, inflation, and altered pain sensitivity. The results of these experiments lead to the conclusion that pre-exposure to shock sensitizes conditional fear responding to similar less intense stressors. This sensitization effect resists exposure therapy (extinction) and amnestic (NMDA antagonist) treatment. The pattern predicts why in PTSD patients, mild stressors cause reactions more appropriate for the original traumatic stressor and why new fears are so readily formed in these patients. This model can facilitate the study of neurobiological mechanisms underlying sensitization of responses observed in PTSD.

Download full-text


Available from: Michael Fanselow, Jan 27, 2015
109 Reads
  • Source
    • "Malfunctions in medial prefrontal cortex ( mPFC ) —amygdala circuit have been identified in patients suffering PTSD , social anxiety disorder ( SAD ) and general anxiety disorder ( GAD ; Schwartz and Rauch , 2004 ; Cottraux , 2005 ; Guyer et al. , 2008 ) . "
    [Show abstract] [Hide abstract]
    ABSTRACT: Altered medial prefrontal cortex (mPFC) and amygdala function is associated with anxiety-related disorders. While the mPFC-amygdala pathway has a clear role in fear conditioning, these structures are also involved in active avoidance. Given that avoidance perseveration represents a core symptom of anxiety disorders, the neural substrate of avoidance, especially its extinction, requires better understanding. The present study was designed to investigate the activity, particularly, inhibitory neuronal activity in mPFC and amygdala during acquisition and extinction of lever-press avoidance in rats. Neural activity was examined in the mPFC, intercalated cell clusters (ITCs) lateral (LA), basal (BA) and central (CeA) amygdala, at various time points during acquisition and extinction, using induction of the immediate early gene product, c-Fos. Neural activity was greater in the mPFC, LA, BA, and ITC during the extinction phase as compared to the acquisition phase. In contrast, the CeA was the only region that was more activated during acquisition than during extinction. Our results indicate inhibitory neurons are more activated during late phase of acquisition and extinction in the mPFC and LA, suggesting the dynamic involvement of inhibitory circuits in the development and extinction of avoidance response. Together, these data start to identify the key brain regions important in active avoidance behavior, areas that could be associated with avoidance perseveration in anxiety disorders.
    Frontiers in Behavioral Neuroscience 09/2015; 9. DOI:10.3389/fnbeh.2015.00249 · 3.27 Impact Factor
  • Source
    • "In preclinical models of PTSD, exposure to uncontrollable traumatic stress leads to enhanced fear conditioning, expression, and interference with extinction (Rau et al., 2005; Baratta et al., 2007, 2008, 2015). Uncontrollable stress triggers a release of serotonin (5-HT) in the brain, specifically in regions known to modulate fear learning and recall including the medial prefrontal cortex (Kawahara et al., 1993; Bland et al., 2003), basolateral amygdala (Kawahara et al., 1993; Amat et al., 1998b) and hippocampus (Amat et al., 1998a). "
    [Show abstract] [Hide abstract]
    ABSTRACT: The capacity to discriminate between safety and danger is fundamental for survival, but is disrupted in individuals with posttraumatic stress disorder (PTSD). Acute stressors cause a release of serotonin (5-HT) in the forebrain, which is one mechanism for enhanced fear and anxiety; these effects are mediated by the 5-HT2C receptor. Using a fear discrimination paradigm where a danger signal conditioned stimulus (CS+) co-terminates with a mild footshock and a safety signal (CS-) indicates the absence of shock, we demonstrate that danger/safety discrimination and fear inhibition develop over the course of 4 daily conditioning sessions. Systemic administration of the 5-HT2C receptor antagonist SB 242084 (0.25 or 1.0mg/kg) prior to conditioning reduced behavioral freezing during conditioning, and improved learning and subsequent inhibition of fear by the safety signal. Discrimination was apparent in the first recall test, and discrimination during training was evident after 3days of conditioning versus 5days in the vehicle treated controls. These results suggest a novel therapeutic use for 5-HT2C receptor antagonists to improve learning under stressful circumstances. Potential anatomical loci for 5-HT2C receptor modulation of fear discrimination learning and cognitive performance enhancement are discussed. Ethical StatementJohn P. Christianson and Allison R. Foilb, the authors, verify that animal research was carried out in accordance with the National Institute of Health Guide for the Care and Use of Laboratory Animals (NIH Publications No. 80-23) and all procedures involving animals were reviewed and approved by the Boston College Animal Care and Use Committee. All efforts were made to limit the number of animals used and their suffering.
    Progress in Neuro-Psychopharmacology and Biological Psychiatry 09/2015; 65. DOI:10.1016/j.pnpbp.2015.08.017 · 3.69 Impact Factor
    • "Mean (+SEM) percentage of freezing during the Context B test on day 3. Animals not shocked in Context B showed minimal levels of freezing in Context B. Animals not shocked in Context A and given one shock in Context B showed moderate levels of freezing while animals shocked in both contexts showed a reliably higher percentage of freezing compared to the other groups. (Adapted from Rau et al. 2005 "
    [Show abstract] [Hide abstract]
    ABSTRACT: In this review, we discuss the usefulness of the distinction between fear and anxiety. The clinical use of the labels is ambiguous, often defining one in terms of the other. We first consider what a useful, objective, and scientifically valid definition would entail and then evaluate several fear/anxiety distinctions that have been made in the neurobiological literature. A strong distinction should specify the difference in conditions that lead to fear versus anxiety. Additionally, fear and anxiety should generate distinct sets of behaviors. Ideally, the two states should be supported by distinguishable neuroanatomical circuits. Such a conceptualization would be consistent with the National Institute of Mental Health's Research Domain Criteria (RDoc). The majority of neurobiological approaches to the fear versus anxiety distinction fail to differentiate the two states in terms of behavior, often using the exact same behavioral measures as indicators. Of the two that do, only Predatory Imminence Theory provides a distinction both in terms of cause and effect. Indeed, that approach provides a ready distinction of anxiety, fear, and panic in terms of both antecedent conditions and response selection rules. Additionally, it appeals to distinct neural circuits to generate these modes of action. © 2015 Perusini and Fanselow; Published by Cold Spring Harbor Laboratory Press.
    Learning & memory (Cold Spring Harbor, N.Y.) 09/2015; 22(9):417-25. DOI:10.1101/lm.039180.115 · 3.66 Impact Factor
Show more