Evolutionary relationships among Aspergillus terreus isolates and their relatives
ABSTRACT Aspergillus terreus is a ubiquitous fungus in our environment. It is an opportunistic human pathogen and economically important as the main producer of lovastatin, a cholesterol lowering drug. Our aim was to examine the genetic variability of A. terreus and closely related species using molecular and analytical techniques. Lovastatin production was examined by HPLC. Lovastatin was produced by seven isolates belonging to the species A. terreus. RAPD analyses were carried out using 25 different random primers. Neighbor-joining analysis of RAPD data (120 characters) resulted in clustering of the A. terreus isolates into distinct groups. Some correlation was observed between lovastatin producing abilities of the isolates and their position on the dendrogram based on RAPD profiles. The internal transcribed spacer region and the 5.8S rRNA gene of A. terreus and related isolates was also sequenced. Phylogenetic analysis of sequence data let us classify the isolates into different clades which mostly correspond to the species Aspergillus terreus, Aspergillus flavipes, Aspergillus niveus, Aspergillus carneus and Aspergillus janus/A. janus var. brevis. Aspergillus allahabadii, A. terreus var. aureus and A. niveus var. indicus belonged to the A. niveus clade, while an Aspergillus isolate previously classified as A. niveus was most closely related to A. flavipes isolates. Aspergillus anthodesmis formed a distinct branch on the tree. Although it was previously suggested based on 28S rDNA sequence data that Aspergillus section Terrei should include A. carneus and A. niveus isolates, phylogenetic analysis of ITS sequences indicate that A. flavipes isolates are more closely related to A. terreus than A. carneus isolates. Our data suggest that sections Terrei and Flavipedes should be merged. However, further loci should be analysed to draw more definite conclusions.
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- "Several recent studies have clearly demonstrated that morphological methods are poor markers of species in the genus Aspergillus, and molecular methods may be useful in species identification  . Phylogenetic analyses of DNA sequences derived from ITS regions and showed the presence of three main clades, which included A. terreus, A. carneus and A. niveus within the section Terrei . However, that study concluded the section Terrei warranted further detailed molecular analysis including more loci and isolates. "
ABSTRACT: RAPD was used to examine the genetic variability among five isolates of Aspergillus terreus spp. Two random primers were selected for the RAPD assay PG01–5' CAGGTGTTGC 3' and PG02–5' CTGGA-CAGAC 3' (Progen Technologies). The characteriza-tion of Aspergillus terreus species have been mostly applied on the basis of morphology, phenotype and physiology. DNA Polymorphisms are based on dif-ferences in DNA sequences and have advantages over protein polymorphisms. But morphological charac-terization besides molecular tools will remain a basic and powerful key in the identification of Aspergillus terreus species. The objective of the present study was to isolate the fungal contaminants from dried grapes and compare the genomic profile of the Aspergillus terreus speices isolated from the dried grapes, through RAPD analysis. In the present study with primer PG 01 four different discriminations was there among the A. terreus isolates. There was a ho-mology of genotype between the isolates 1 & 3. And with primer PG 02 four different discriminations were there and there was a homology between 1 & 3. The predominant type was type I in primer I & II. The other isolates belonged to 2, 3 and 4. No similar-ity was detected for isolates 3, 4 and 5 indicating great genomic diversity of A. terreus.Advances in Bioscience and Biotechnology 01/2010; 1(04):345. DOI:10.4236/abb.2010.14046
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- "(B) Wet mount preparation with lactophenol cotton blue shows a smooth colorless conidiophore and a small vesicle covered by biserate phialides with round smooth conidia. soil-related species, being frequently resistant to AMB and having endemic character in Tyrol (Iwen et al., 1998; Lass- Flörl et al., 2005; Varga et al., 2005; Wadhwani and Srivastava, 1984). Species of Aspergillus other than A. fumigatus have been increasingly isolated in some institutions, and the most important species include Aspergillus flavus, Aspergillus niger, and A. terreus (Cornet et al., 2002; Lass-Flörl et al., 2000; Perfect et al., 2001; Steinbach et al., 2004). "
ABSTRACT: Aspergillus niveus is a species uncommon in clinical samples, and to date, invasive fungal infections caused by this fungal pathogen have not been described. This is the 1st report on a pulmonary breakthrough aspergillosis caused by A. niveus in a 21-year-old woman after allogeneic hematopoietic stem cell transplantation for Fanconi anemia.Diagnostic Microbiology and Infectious Disease 09/2008; 62(3):336-9. DOI:10.1016/j.diagmicrobio.2008.06.012 · 2.57 Impact Factor
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ABSTRACT: Under the NIST ATP-sponsored “precision optoelectronics assembly” program, Adept Technology, Boeing, Dresser Industries, National Center for Manufacturing Sciences, New Jersey Institute of Technology, SDL, and SRI International, are collaboratively developing key enabling technologies for low-cost, flexible, automated assembly of optoelectronics systems. Midway through the program the Consortium has developed a prototype assembly platform. Precision optoelectronics assembly processes for beta-site testing have been definedLasers and Electro-Optics Society Annual Meeting, 1997. LEOS '97 10th Annual Meeting. Conference Proceedings., IEEE; 12/1997