Salmonella typhimurium grown in iron-rich media, inactivated with ferric chloride and adjuvanted with homologous bacterial DNA is potent and efficacious vaccine in mice.

Abstract

The present study describes our attempt to construct a novel vaccine formulation that affords full protection against murine typhoid under experimental conditions. Ferric chloride, 100mM, as inactivating agent, bacterial growth under iron-rich conditions and homologous bacterial DNA as adjuvant were used for construction of the experimental Salmonella typhimurium vaccine. The vaccine inoculated twice at 2 weeks interval in Swiss albino mice elicited statistically significant IgG levels when compared with non-adjuvanted and other control groups. All the mice inoculated with the novel vaccine withstood challenge with 50 LD(50) dose of S. typhimurium strain St 585. No significant safety problems were found in mice.