Article

[Rapid amplification of 3' cDNA end of Suaeda liaotungensis betaine aldehyde dehydrogenase using one-step PCR].

Biology Department of Liaoning Normal University, Dalian 116029, China.
Hereditas (Beijing) 04/2002; 24(2):179-81. pp.179-81
Source: PubMed

ABSTRACT Based on part of a known cDNA sequence of Suaeda liaotungensis betaine aldehyde dehydrogenase, we successfully cloned the 3' cDNA end of S. lianotungensis betaine aldehyde dehydrogenase using one-step PCR with a gene-specific primer and universal primer. Compared with the typical 3' RACE, one-step PCR is rapid, simple and inexpensive. It is very rapid to amplify an unknown cDNA 3' end using this method.

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Keywords

3' cDNA end
 
inexpensive
 
known cDNA sequence
 
S. lianotungensis betaine aldehyde dehydrogenase
 
Suaeda liaotungensis betaine aldehyde dehydrogenase
 
typical 3' RACE
 
universal primer
 
unknown cDNA 3' end