Charged polypeptide vesicles with controllable diameter

Contribution from the Materials Department, University of California at Santa Barbara, Santa Barbara, California 93106, USA.
Journal of the American Chemical Society (Impact Factor: 11.44). 10/2005; 127(35):12423-8. DOI: 10.1021/ja053557t
Source: PubMed

ABSTRACT We report the preparation and characterization of charged, amphiphilic block copolypeptides that form stable vesicles and micelles in aqueous solution. Specifically, we prepared and studied the aqueous self-assembly of a series of poly(L-lysine)-b-poly(L-leucine) block copolypeptides, KxLy, where x ranged from 20 to 80 and y ranged from 10 to 30 residues, as well as the poly(L-glutamatic acid)-b-poly(L-leucine) block copolypeptide, E60L20. Furthermore, the vesicular assemblies show dynamic properties, indicating a high degree of membrane fluidity. This characteristic provides stimuli-responsive properties to the vesicles and allows fine adjustment of vesicle size using liposome-based extrusion techniques. Vesicle extrusion also provides a straightforward means to trap solutes, making the vesicles promising biomimetic encapsulants.

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