[Male genital tract infection: the point of view of the bacteriologist].

Laboratoire de biologie médicale Magenta, 41, boulevard de Magenta, 75010 Paris, France.
Gynécologie Obstétrique & Fertilité (Impact Factor: 0.55). 10/2005; 33(9):691-7. DOI: 10.1016/j.gyobfe.2005.07.008
Source: PubMed

ABSTRACT Male genital tract infection and inflammation have been associated to 8 to 35% of male infertility cases in various studies. Their investigation is part of a multi-disciplinary process including new techniques as DNA integrity study. Bacterial seminal infection can cause transient or chronic persistent inflammation, and the microbiological investigations, as well as leukospermia, secretory chlamydial IgA and inflammatory cytokines help to approach the responsibility of inflammation in infertility or pathological condition, leading to antibiotic and anti-inflammatory treatment. In Assisted Reproductive Techniques (ART), bacteriospermia must be eradicated for a safe semen preparation to inseminate or to fertilize oocytes. Leukocytes cannot be completely eliminated by sperm preparation and the presence of antibiotics and antioxydants in the culture media is questionned.

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    ABSTRACT: One hundred asthenospermic seminal fluid specimens were collected from 100 infertile males who referred to Babylon maternity and children hospital-infertility center. It was found that 70 seminal fluid specimens had asthenospermia with leukocytospermia (First group). The rest 30 seminal fluid specimens had asthenospermia without leukocytospermia (Second group). Regarding semen cultures the results showed that 61(87.1%) of specimens of First group revealed positive bacterial culture, whereas 9(12.9%) specimens of First group showed no bacterial growth even after incubation of 48 hours. All semen specimens of Second group revealed negative bacterial culture. Gram positive bacteria constituted 44(62.9%) while gram negative bacteria constituted 26(37.1%) of isolates. Coagulase negative staphylococci (CoNS) represented by Staphylococcus epidermides and Staphylococcus saprophyticus) were the common type of bacterial isolates 25(35.7%) followed by Staphylococcus aureus 19(27.2%), Escherichia coli 12(17.1%), Enterobacter aerogenes 8(11.4%), Acinetobacter spp 4(5.7%) and Moraxella spp 2(2.9%). The virulence factors of bacterial isolates were investigated. The results showed that all S. aureus isolates, 18(72%) isolates of CoNS and 5(41.7%) of E. coli isolates and 4(50%) of E. aerogenes isolates produce hemolysin. Colonization factor antigens (CFA/�) were detected in all isolates of S. aureus, CoNS, E. coli, E. aerogenes, Acinetobacter spp. and Moraxella spp. (CFA/�) were expressed in 10(52.6%) isolates of S. aureus, 8(32%) isolates of CoNS, 8(66.7%) isolates of E. coli, 6(75%) isolates of E. aerogenes, 2(50%) isolates of Acinetobacter spp. and 1(50%) isolate of Moraxella spp. Lipase produced by 15(78.9%) and 7(28%) isolates of S. aureus and CoNS isolates respectively, while 9(75%) isolates of E. coli , 7(87.5%) isolates of E. aerogenes and 1(50%) isolate of Moraxella spp. produce lipase. Only 7(36.8%) isolates of S. aureus and 5(41.7%) isolates of E. coli were found to be protease producers. The effects of some antibiotics on bacterial isolates were investigated. The results showed that, the bacterial isolates were highly susceptible to imipenem, meropenem and ciprofloxacin whereas exhibited moderate resistance to amikacin, gentamycin and norfloxacin. On the other hand bacterial isolates revealed high rate of resistance to amoxicillin, ceftizoxime, ceftazidime, cefamandole, cefepime, amoxicillin–clavulanic acid and tobramycin.
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    ABSTRACT: The role of inflammation and/or infection of the male accessory sex glands is very important for the potential effects that these conditions have on male fertility. The clinical Andrologist should be aware of the pathophysiological role of the main determinants of sperm damage when these conditions occur, in particular seminal leukocytes, oxidative stress, and cytokines. In addition, it is important to have a good knowledge of the methodologies to be used in the clinical practice. This article summarizes the methods used to look for and to identify the microorganisms responsible for male urogenital tract infections. These include sperm culture, urine culture, urethral swab, Meares-Stamey test, and balanopreputial swab. In the last part, we discussed the role of human papillomavirus (HPV) infection in male infertility.
    Journal of Medical Microbiology 09/2013; · 2.30 Impact Factor
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    ABSTRACT: OBJECTIVE: To assess the relationship between mycoplasma infection and human infertility, we determined the concordance of Ureaplasma urealyticum (UU) and Mycoplasma hominis (MH) detection in infertile and fertile couples, and assessed semen parameters in both groups. METHODS: Fifty infertile couples without a female factor attending a fertility clinic and 48 fertile couples were randomly screened for UU and MH. The concordance between partners was compared between the fertile and infertile groups. Semen and endocervical specimens were evaluated using the commercially available Mycofast Evolution2 test. RESULTS: UU was detected in 24 semen specimens (48%) from the infertile men, in 12 specimens from fertile men (25%), in 20 endocervical specimens from infertile women (40%), and 11 from fertile women (22.9%). UU was detected higher in infertile men than in fertile men (P = .022). The concordance of UU was higher in infertile couples (32%) than in fertile couples (12.5%, P = .022). The concordance of MH between male and female partners in the 2 groups did not differ significantly. The mean values of total motility, progressive motility, normal morphology, vitality, and total motile sperm count were significantly lower in sperm from infertile men than from fertile men. Progressive motility and vitality were significantly lower in UU-positive men than in men without UU, and low total motility and total motile sperm count were significantly related to the presence of MH. CONCLUSION: Clinicians should consider the roles of UU and MH in infertility and routinely screen infertile couples for the presence of these mycoplasma species.
    Urology 04/2013; · 2.42 Impact Factor