[Male genital tract infection: the point of view of the bacteriologist].
ABSTRACT Male genital tract infection and inflammation have been associated to 8 to 35% of male infertility cases in various studies. Their investigation is part of a multi-disciplinary process including new techniques as DNA integrity study. Bacterial seminal infection can cause transient or chronic persistent inflammation, and the microbiological investigations, as well as leukospermia, secretory chlamydial IgA and inflammatory cytokines help to approach the responsibility of inflammation in infertility or pathological condition, leading to antibiotic and anti-inflammatory treatment. In Assisted Reproductive Techniques (ART), bacteriospermia must be eradicated for a safe semen preparation to inseminate or to fertilize oocytes. Leukocytes cannot be completely eliminated by sperm preparation and the presence of antibiotics and antioxydants in the culture media is questionned.
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ABSTRACT: The assisted reproductive techniques aimed to assist infertile couples have their own offspring carry significant risks of passing on molecular defects to next generations.Journal of Assisted Reproduction and Genetics 08/2014; · 1.82 Impact Factor
Conference Paper: 2rd International Science Congress (ISC-2012)2rd International Science Congress (ISC-2012); 12/2012
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ABSTRACT: One hundred asthenospermic seminal fluid specimens were collected from 100 infertile males who referred to Babylon maternity and children hospital-infertility center. It was found that 70 seminal fluid specimens had asthenospermia with leukocytospermia (First group). The rest 30 seminal fluid specimens had astheno-spermia without leukocytospermia (Second group). Regarding semen cultures the results showed that 61(87.1%) of specimens of First group revealed positive bacterial culture, whereas 9(12.9%) specimens of First group showed no bacterial growth even after incubation of 48 hours. All semen specimens of Second group revealed negative bacterial culture. Gram positive bacteria constituted 44(62.9%) while gram negative bacteria constituted 26(37.1%) of isolates. Coagulase negative staphylococci (CoNS) represented by Staphylococcus epidermides and Staphylococcus saprophyticus) were the common type of bacterial isolates 25(35.7%) followed by Staphylococcus aureus 19(27.2%), Escherichia coli 12(17.1%), Enterobacter aerogenes 8(11.4%), Acinetobacter spp. 4(5.7%) and Moraxella spp 2(2.9%). The virulence factors of bacterial isolates were investigated. The results showed that all S. aureus isolates, 18(72%) isolates of CoNS and 5(41.7%) of E. coli isolates and 4(50%) of E. aerogenes isolates produce hemolysin. Colonization factor antigens (CFA/Ш) were detected in all isolates of S. aureus, CoNS, E. coli, E. aerogenes, Acinetobacter spp. and Moraxella spp. (CFA/Ι) were expressed in 10(52.6%) isolates of S. aureus, 8(32%) isolates of CoNS, 8(66.7%) isolates of E. coli, 6(75%) isolates of E. aerogenes, 2(50%) isolates of Acinetobacter spp. and 1(50%) isolate of Moraxella spp. Lipase produced by 15(78.9%) and 7(28%) isolates of S. aureus and CoNS isolates respectively, while 9(75%) isolates of E. coli , 7(87.5%) isolates of E. aerogenes and 1(50%) isolate of Moraxella spp. produce lipase. Only 7(36.8%) isolates of S. aureus and 5(41.7%) isolates of E. coli were found to be protease producers. The effects of some antibiotics on bacterial isolates were investigated. The results showed that, the bacterial isolates were highly susceptible to imipenem, meropenem and ciprofloxacin whereas exhibited moderate resistance to amikacin, gentamycin and norfloxacin. On the other hand bacterial isolates revealed high rate of resistance to amoxicillin, ceftizoxime, ceftazidime, cefamandole, cefepime, amoxicillin–clavulanic acid and tobramycin.Int. Res. J. Medical Sci. 02/2013; 1(1):1-6.