Male genital tract infection: The point of view of the bacteriologist

Laboratoire de biologie médicale Magenta, 41, boulevard de Magenta, 75010 Paris, France.
Gynécologie Obstétrique & Fertilité (Impact Factor: 0.52). 10/2005; 33(9):691-7. DOI: 10.1016/j.gyobfe.2005.07.008
Source: PubMed


Male genital tract infection and inflammation have been associated to 8 to 35% of male infertility cases in various studies. Their investigation is part of a multi-disciplinary process including new techniques as DNA integrity study. Bacterial seminal infection can cause transient or chronic persistent inflammation, and the microbiological investigations, as well as leukospermia, secretory chlamydial IgA and inflammatory cytokines help to approach the responsibility of inflammation in infertility or pathological condition, leading to antibiotic and anti-inflammatory treatment. In Assisted Reproductive Techniques (ART), bacteriospermia must be eradicated for a safe semen preparation to inseminate or to fertilize oocytes. Leukocytes cannot be completely eliminated by sperm preparation and the presence of antibiotics and antioxydants in the culture media is questionned.

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    • "In birds, E. coli could be transmitted to the progeny through copulation. Microorganisms present in the seminal fluid or attached to spermatozoa are transmitted to females (Barnes and Gross, 1997; Jacobs et al., 1978; Poiani, 2010) causing reproductive tract infections (Askienazy-Elbhar, 2005) and embryonic mortality (Reid et al., 1961). Despite the numerous publications concerning sperm infection, to date, there are no data on the potential impact that could be related to resistant E. coli contamination. "
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    Animal reproduction science 06/2015; 159. DOI:10.1016/j.anireprosci.2015.06.021 · 1.51 Impact Factor
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    • "False negatives may result from the interference of pathogenic bacteria in the culture medium due to bactericide production by Gram-positive bacteria. To prevent false negatives, it is necessary to dilute the seminal fluid with saline before plating it onto agar media (Askienazy-Elbhar, 2005; De Francesco et al., 2011; Boitrelle et al., 2012). "
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    ABSTRACT: The role of inflammation and/or infection of the male accessory sex glands is very important for the potential effects that these conditions have on male fertility. The clinical Andrologist should be aware of the pathophysiological role of the main determinants of sperm damage when these conditions occur, in particular seminal leukocytes, oxidative stress, and cytokines. In addition, it is important to have a good knowledge of the methodologies to be used in the clinical practice. This article summarizes the methods used to look for and to identify the microorganisms responsible for male urogenital tract infections. These include sperm culture, urine culture, urethral swab, Meares-Stamey test, and balanopreputial swab. In the last part, we discussed the role of human papillomavirus (HPV) infection in male infertility.
    Journal of Medical Microbiology 09/2013; 63(Pt_1). DOI:10.1099/jmm.0.062968-0 · 2.25 Impact Factor
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    • "The detection of C trachomatis in 4 patients and M genitalium in 1 patient only in semen may indicate that these organisms are harboured in the epididymis or seminal vesicles. Various studies conducted in industrialized countries proved the high prevalence of C trachomatis, genital ureaplasmas, and genital mycoplasmas among male partners of infertile couples and its important role in some cases in infertility (Upadhyaya et al, 1984; Paavonen and Wolner-Hassen, 1989; Vigil et al, 2002; Askienazy-Elbhar, 2005; Wang et al, 2006). Yet, in developing countries, the situation is not always clear. "
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