Advanced granulomatous lesions in Mycobacterium bovis-infected cattle are associated with increased expression of type I procollagen, gammadelta (WC1+) T cells and CD 68+ cells.
ABSTRACT The pathognomonic characteristic of tuberculosis (TB) is the formation of a tuberculous granuloma. The objective of this study was to classify lymph node granulomas from experimentally infected calves into different histopathological stages and characterize them further by studying cell types and markers of fibrosis associated with each of the stages. Four stages of granuloma were identified and mRNA and protein expression for cell markers, cytokines and pro-fibrotic markers were studied by immunohistochemistry (IHC) and in-situ hybridization (ISH). In advanced stage granulomas, there was an increase in the expression of TGF-beta, and of type I procollagen as demonstrated by IHC and ISH. As the granulomas advanced, there were fewer CD3+T cells and they tended to be more prominent towards the periphery of the lesions, with a steady increase in the number of CD68+ cells and gammadelta (WC1+) T cells. Granuloma classification and application of cell cytokine markers will assist in improving understanding of the pathogenesis of bovine TB and may help to identify the immunopathology of active disease versus contained or inactive disease. Such disease correlates may help to inform the development of improved diagnostic methods and support vaccine development programmes.
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- "Positive and negative control tissues were also included. Stained sections were examined for typical tuberculous lesions and AFB based on the TB lesion pattern previously established in cattle and goats (Guti errez-Cancela, 1996; Wangoo et al., 2005; Garc ıa Castro, 2007). The lesions (granulomas) were classified on the basis of (i) extension, (ii) their intensity and inflammatory cell type, (iii) mineralization and calcification and (iv) their degree of encapsulation . "
ABSTRACT: Bovine tuberculosis (TB) infection is infrequently diagnosed in sheep. Most reports are from single individual cases or flock outbreaks. However, in Spain several outbreaks have been reported recently, all of which had epidemiological links with TB-infected cattle herds. A total of 897 sheep suspected of being infected with TB and belonging to 23 flocks cohabiting with TB-infected cattle herds and/or goats were tested between 2009 and 2013 in Galicia (north-western Spain), using pathological, immunological and molecular techniques. Of these, 50.44% were positive by culture, 83.23% by histopathology and 24.92%, 4.86% and 59.42% by single intradermal tuberculin test (SITT), interferon-γ and ELISA, respectively. Results suggest that in circumstances akin to those in our study, sheep may be considered as a potential source of TB. We conclude that under similar conditions, serious consideration should be given to TB testing sheep, as they may represent a potential risk to other susceptible co-habiting species. The SITT and ELISA are recommended as the simplest and most cost-effective initial approaches for the diagnosis of TB in sheep under field conditions. However, when possible, interferon-γ should be applied to increase sensitivity.Transboundary and Emerging Diseases 01/2015; DOI:10.1111/tbed.12325 · 3.12 Impact Factor
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- "Determination of the percentage of the lungs containing lesions has been determined by radiography (Waters et al., 2007). The severity of lesions has also been estimated from histopathological examination and scores of I–IV have been used to classify the types of granulomas present in lesions (Wangoo et al., 2005). "
ABSTRACT: Tuberculosis either caused by Mycobacterium bovis or M. caprae is a significant burden to agricultural industries worldwide. Vaccination of domestic ruminant species such as cattle and goats constitutes a potential tool to support disease control. This review will discuss recent progress made to develop tuberculosis vaccines against domestic ruminants as well as approaches to differentiate vaccinated and infected animals (DIVA) and biomarker discovery studies.Research in Veterinary Science 05/2014; DOI:10.1016/j.rvsc.2014.04.015 · 1.51 Impact Factor
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- "Scoring of haematoxylin and eosin stained lesions Formalin-fixed, paraffin-embedded vaccine and infection site lesions were sectioned (4 lm) and stained with haematoxylin and eosin for histologic evaluation and lesion scoring. Haematoxylin and eosin stained vaccine and infection site lesions were individually scored using a scoring system adapted from previous studies (Wangoo et al. 2005; Johnson et al. 2006; Lei et al. 2008). Scores of 0–3 were assigned for each parameter to reflect the degree and organization within each lesion. "
ABSTRACT: The characteristic lesion in bovine tuberculosis is well-organized respiratory granulomas. This is typically associated with a strong T-helper 1 biased cell-mediated immune response and eventual containment of the infection. In bovine paratuberculosis, the classic lesion is unorganized granulomatous intestinal inflammation. Clinical paratuberculosis is associated with a T-helper 2 biased humoral immune response and eventual death because of inability of the host to contain the infection. Recent reports have suggested that gamma-delta (gammadelta) T cells play a significant role in granuloma development and/or maintenance during initial stages of infection and may influence the subsequent adaptive immune response. The objective of this study was to use an in vivo bovine model to evaluate gammadelta T cells during the early host immune response to mycobacterial infection. We used immunofluorescent staining, hyperspectral microscopy, and computerized assisted morphometry to evaluate staining and distribution of gammadelta T cells during development of organized and unorganized granulomas. Our data suggest that bovine gammadelta T cell subsets are differentially recruited to early infection sites, and may be instrumental during the initial antimycobacterial host immune response as well as for granuloma organization.International Journal of Experimental Pathology 09/2009; 90(6):587-97. DOI:10.1111/j.1365-2613.2009.00679.x · 2.05 Impact Factor