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Advanced granulomatous lesions in Mycobacterium bovis-infected cattle are associated with increased expression of type I procollagen, gammadelta (WC1+) T cells and CD 68+ cells.

Department of Pathology, Veterinary Laboratories Agency-Weybridge, Addlestone, Surrey, KT15 3NB, UK.
Journal of Comparative Pathology (Impact Factor: 1.1). 12/2005; 133(4):223-34. DOI: 10.1016/j.jcpa.2005.05.001
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ABSTRACT The pathognomonic characteristic of tuberculosis (TB) is the formation of a tuberculous granuloma. The objective of this study was to classify lymph node granulomas from experimentally infected calves into different histopathological stages and characterize them further by studying cell types and markers of fibrosis associated with each of the stages. Four stages of granuloma were identified and mRNA and protein expression for cell markers, cytokines and pro-fibrotic markers were studied by immunohistochemistry (IHC) and in-situ hybridization (ISH). In advanced stage granulomas, there was an increase in the expression of TGF-beta, and of type I procollagen as demonstrated by IHC and ISH. As the granulomas advanced, there were fewer CD3+T cells and they tended to be more prominent towards the periphery of the lesions, with a steady increase in the number of CD68+ cells and gammadelta (WC1+) T cells. Granuloma classification and application of cell cytokine markers will assist in improving understanding of the pathogenesis of bovine TB and may help to identify the immunopathology of active disease versus contained or inactive disease. Such disease correlates may help to inform the development of improved diagnostic methods and support vaccine development programmes.

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    • "Positive and negative control tissues were also included. Stained sections were examined for typical tuberculous lesions and AFB based on the TB lesion pattern previously established in cattle and goats (Guti errez-Cancela, 1996; Wangoo et al., 2005; Garc ıa Castro, 2007). The lesions (granulomas) were classified on the basis of (i) extension, (ii) their intensity and inflammatory cell type, (iii) mineralization and calcification and (iv) their degree of encapsulation . "
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    • "Determination of the percentage of the lungs containing lesions has been determined by radiography (Waters et al., 2007). The severity of lesions has also been estimated from histopathological examination and scores of I–IV have been used to classify the types of granulomas present in lesions (Wangoo et al., 2005). "
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    • "Scoring of haematoxylin and eosin stained lesions Formalin-fixed, paraffin-embedded vaccine and infection site lesions were sectioned (4 lm) and stained with haematoxylin and eosin for histologic evaluation and lesion scoring. Haematoxylin and eosin stained vaccine and infection site lesions were individually scored using a scoring system adapted from previous studies (Wangoo et al. 2005; Johnson et al. 2006; Lei et al. 2008). Scores of 0–3 were assigned for each parameter to reflect the degree and organization within each lesion. "
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