Progesterone modulation of inflammatory cytokine production in a fetoplacental artery explant model.
ABSTRACT The purpose of this study was to determine if progesterone has an effect on fetoplacental artery production of inflammatory cytokines.
Chorionic plate arteries were dissected from 5 placentas obtained from normal pregnancies after delivery at term. Arteries were incubated in Dulbecco's modified Eagle's medium (DMEM) alone, DMEM and lipopolysaccharide (LPS), DMEM with progesterone (P4), and DMEM with P4 and LPS. Samples of the tissue culture media were collected and evaluated for interleukin 6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), and interleukin-10 (IL-10) by immunoassay.
There was a significant decrease in the production of IL-6 in P4-exposed fetoplacental arteries after LPS stimulation (P < .001). IL-10 and TNF-alpha levels were similar in control and treatment groups after LPS exposure.
Pretreating fetoplacental arteries with P4 significantly decreased the production of IL-6 after LPS stimulation without altering the production of TNF-alpha or IL-10.
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ABSTRACT: Infection of human fetal membranes elicits secretion of pro-inflammatory modulators through its innate immune capacities. We investigated the effect of lipopolysacharide (LPS) and progesterone (P4) upon expression of TLR-4/MyD88, TNFα, IL-6, IL-8, IL-10, and HBD2 on the human amniotic epithelium. Explants of the human amniotic epithelium were pre-treated with 0.01, 0.1, and 1.0 μm of P4; then cotreated with 1000 ng/mL LPS. TLR-4 was immuno-detected, and concentrations of MyD88, TNFα, IL-6, IL-8, IL-10, and HBD2 were quantified by ELISA. P4 significantly reduced the expression of LPS-induced TLR-4/MyD88. LPS increased the concentrations of TNFα, IL-6, IL-8, IL-10, and HBD2 by factors of 30-, eight, three, three, and fivefold, respectively. P4 at 1.0 μm was the most effective dose to blunt the secretion of TNFα, IL-6, and HBD-2. RU-486 blocks the effect of P4. P4 inhibited LPS-induced TLR-4/MyD88 and pro-inflammatory factors in the human amniotic epithelium. These results could explain partially how P4 can protect the amniotic region of fetal membranes and generate a compensatory mechanism that limits the secretion of pro-inflammatory modulators, which could jeopardize the immune privilege during pregnancy.American Journal Of Reproductive Immunology 10/2013; · 3.32 Impact Factor
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ABSTRACT: Bessel beams can be used as optical tweezers, to trap and manipulate small particles, including biological specimens. Here we demonstrate the use of such beams to trap and manipulate particles simultaneously that may reside in completely separate sample chambers, separated by distances that preclude trapping with a Gaussian beam. This also demonstrates another property of the Bessel beam, in that since it is a set of rings it can trap both low and high refractive index particles. The distance behind the particle that the Bessel beam reconstructs is dependent on the properties of the particle, and this may be useful in cell characterisation. We also demonstrate the generation of more complex patterns of nondiffracting light beams, by using interfering Bessel beams. We generate these by using a Mach-Zender interferometer in which each of the arms has a Laguerre-Gaussian beam of differing handedness.01/2003;
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ABSTRACT: Abstract Background: The use of 17-alpha-hydroxyprogesterone caproate (17P) has been shown to reduce preterm delivery in women who have had a prior preterm birth. The role of 17P in women with arrested preterm labor is less certain AIMS: To compare the preterm birth rate and neonatal outcome in women with arrested preterm labor randomized to receive 17P or placebo. Materials and Methods: Women with arrested preterm labor were randomized to weekly injections of either 17P (250mg), or placebo. Maternal and neonatal outcome were evaluated. Results: Forty-five singleton pregnancies were randomized after successful tocolysis; 22 received 17P while 23 got placebo. Gestational age at delivery (p=.067) and the interval from treatment to delivery (p=.233) were not affected by 17P. Significantly less women in the 17P group delivered at <34 weeks (14 vs. 21, p=.035). There was also a significant reduction in the risk of neonatal sepsis (p=0.047) and gr III/IV intraventricular hemorrhage (p=.022) in the 17P group. Conclusion: In this study, 17P did not delay the interval to delivery after successful preterm labor, but births <34 weeks as well as neonatal sepsis and intraventricular hemorrhage were reduced by 17P treatment.The journal of maternal-fetal & neonatal medicine: the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians 02/2014; · 1.36 Impact Factor