To study the chemical characteristic, to identify the different forms and to establish the new standard for the quality control of Cortex Magnoliae Officinalis.
HPLC method was used with acetonitrile-water (63:37) as the mobile phase at room temperature. The chromatographic column was Lichrospher 100 RP-18e (4.6 mm x 250 mm, 5 microm). The flow rate was 1 mL x min(-1), and the detection wavelength was 294 nm. The chromatograms of 45 individuals from 13 seed resources of Cortex Magnolia Officinalis were recorded. The chemical characteristics analysis and comparability' s calculation of seed resources were made.
It was proposed that the area ratio of peak 5 to 6 (characteristic I) and the area ratio of peak 5 and 6 to the total peak areas (characteristic II) are the identification characteristics for different seed resources of Cortex Magnoliae Officinalis.
This method can be used effectively to identify the high quality seed resource of Cortex Magnoliae Officinalis.
[Show abstract][Hide abstract] ABSTRACT: Magnoliae officinalis cortex (MOC), derived from Magnolia officinalis and its variation M. officinalis var. biloba, is an important traditional Chinese medicine. In our previous work, 11 hydrophilic ingredients of MOC were isolated and
structurally elucidated and four, namely syringin (SG), magnoloside A (MA), magnoloside B (MB) and magnoflorine (MF), showed
bioactive effects. Herein, we describe an HPLC–DAD method for the simultaneous quantitative determination of MA, MB, MF and
SG in MOC for the first time. The chromatographic separation of samples was performed on an Agilent Zorbax SB-C18 column (250
× 4.6 mm i.d., 5 µm) by gradient elution with water–acetic acid (pH 3.0) and methanol at a flow rate of 1.0 mL/min. The wavelengths
were set at 265 nm for MF and SG, and 328 nm for MA and MB. The average recovery of the four compounds was from 97.63 to 103.84%.
Nearly 100 MOC samples harvested from eight habitats were analyzed in which the contents of the tested compound varied in
the range of 0.016–0.350% (MF), 0.010–0.337% (SG), 0.017–3.009% (MB) and 0.077–2.529% (MA). The analysis also indicated that
MOC contains a significant amount of phenylethanoid glycosides. This was an unexpected finding because previously lignan was
considered to be the main component of MOC.
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