Heat Shock Protein 32 in Human Peripheral Blood Mononuclear Cells: Effect of Aging and Inflammation

Geriatric Unit, Academic Hospital, Vrije Universiteit Brussel, Belgium.
Journal of Clinical Immunology (Impact Factor: 2.65). 10/2005; 25(5):405-17. DOI: 10.1007/s10875-005-5361-y
Source: PubMed

ABSTRACT The purpose of the present study was to assess the influence of age and acute infection on the production of Hsp32 in human peripheral blood cells, using flow cytometry. Thirty-five controls and 31 patients with acute infection participated. We found that the age and inflammatory status correlated positively with Hsp32 levels in both heat shocked (HS) and non-HS monocytes and lymphocytes. In addition, the HS response of Hsp32 was different in these peripheral blood cells; whereas HS exerted an up-regulation in the levels of Hsp32 in monocytes, a significant decrease in Hsp32 levels was noticed for lymphocytes. We found significant relationships between circulating C-reactive protein as well as interleukin-6 and the levels of Hsp32 in cells. We conclude that Hsp32 is up-regulated in the elderly as well as in individuals with inflammation, and that the HS response of Hsp32 is different in monocytes as compared to lymphocytes.

  • [Show abstract] [Hide abstract]
    ABSTRACT: Providing food, energy and materials for the rising global population is a challenge which is compounded by increased pressure on natural resources such as land, water and fossil sources of raw materials. Greenhouse gas (GHG) emissions from human activities have increased with industrial development and population expansion, and it is anticipated that resulting climate change might further limit agricultural productivity, through changes to weather patterns and global availability/distribution of agriculturally productive land. Growing crops as feedstocks for industrial uses is seen as one way of reducing GHG emissions and dependency on fossil resources. However, determining the extent to which the development of crops for industrial use will effect GHG balances and provide for a more energy efficient manufacturing system requires the development and use of appropriate calculation methodologies.Research at the Porter Institute has identified over 250 different scenarios for bioenergy production systems using commodity crops. In order to rationalise this complexity and diversity, a modular approach to Life Cycle Assessment (LCA) and sustainability analysis has been taken. This allows characterisation of discrete sections of supply chains and enables comparisons to be made between different crop production systems, different process systems and different end product uses. The purposes of this paper are to introduce the concepts of biofuel GHG and sustainability metrics, to introduce the approach taken by our organization and to use the example of UK grown willow in a lignocellulosic ethanol production system to demonstrate how GHG emission outcomes can be reviewed for “new” crops and technologies.The results show a range of variation, in both growing and process systems and how outcomes such as energy and GHG balances can be affected by various activities.LCA methodologies provide data to inform governments and industry of the potential specific supply chains may have for energy and GHG saving. However, methodological approaches can also affect assessment outcomes. Unresolved issues in LCA methodology must also be evaluated e.g. impacts resulting from land use change. Sustainability assessments of crop growing systems, irrespective of the end use, also assist in the assessment of environmental impacts of supply chains. However, it is critical that data continue to be collected, analysed and reviewed, to ensure that the most appropriate crops are grown and processed for the most appropriate end use.
    Industrial Crops and Products 09/2011; 34(2):1332-1339. DOI:10.1016/j.indcrop.2010.12.002 · 3.21 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The present review of published data and the authors’ own results addresses the role of heat shock proteins in the regulation of cell and tissue homeostasis and considers the decrease in their expression levels as one of the main factors of aging. Heat shock proteins are involved in the regulation of proliferation, apoptosis, and differentiation of cells, as well as in that of intracellular homeostasis, and, therefore, play a substantial role in maintaining the activity of the immune, cardiovascular, and other systems of the organism. These proteins are also implied in the development of atherosclerosis, myocardial infarction, ischemic stroke, and other diseases accompanied by thrombotic complications. The use of short peptides provides an opportunity to restore and normalize the expression of heat shock proteins, which probably accounts for the antistress and geroprotective activity of these peptides.
    07/2012; 2(3). DOI:10.1134/S2079057012030071
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Heat shock proteins (HSPs) are molecular chaperones involved in protein folding, assembly and transport, and which play critical roles in the regulation of cell growth, survival and differentiation. We set out to test the hypothesis that HSP27 protein is expressed in the human testes and its expression varies with the state of spermatogenesis. HSP27 expression was examined in 30 human testicular biopsy specimens (normal spermatogenesis, maturation arrest and Sertoli cell only syndrome, 10 cases each) using immunofluorescent methods. The biopsies were obtained from patients undergoing investigations for infertility. The seminiferous epithelium of the human testes showing normal spermatogenesis had a cell type-specific expression of HSP27. HSP27 expression was strong in the cytoplasm of the Sertoli cells, spermatogonia, and Leydig cells. Alternatively, the expression was moderate in the spermatocytes, weak in the spermatids and absent in the spermatozoa. In testes showing maturation arrest, HSP27 expression was strong in the Sertoli cells, weak in the spermatogonia, and spermatocytes. It was absent in the spermatids and Leydig cells. In Sertoli cell only syndrome, HSP27 expression was strong in the Sertoli cells and absent in the Leydig cells. We report for the first time the expression patterns of HSP27 in the human testes and show differential expression during normal spermatogenesis, indicating a possible role in this process. The altered expression of this protein in testes showing abnormal spermatogenesis may be related to the pathogenesis of male infertility.
    Cell Biology International 10/2008; 32(10-32):1247-1255. DOI:10.1016/j.cellbi.2008.07.009 · 1.64 Impact Factor