Ultrastructural Morphometrical and Immunocytochemical Analyses of Hepatocyte Nuclei from Mice Fed on Genetically Modified Soybean

Istituto di Istologia e Analisi di Laboratorio, via Zeppi s n, University of Urbino, Italy.
Cell Structure and Function (Impact Factor: 1.68). 09/2002; 27(4):173-80. DOI: 10.1247/csf.27.173
Source: PubMed


No direct evidence that genetically modified (GM) food may represent a possible danger for health has been reported so far; however, the scientific literature in this field is still quite poor. Therefore, we carried out an ultrastructural morphometrical and immunocytochemical study on hepatocytes from mice fed on GM soybean, in order to investigate eventual modifications of nuclear components of these cells involved in multiple metabolic pathways related to food processing. Our observations demonstrate significant modifications of some nuclear features in GM-fed mice. In particular, GM fed-mice show irregularly shaped nuclei, which generally represents an index of high metabolic rate, and a higher number of nuclear pores, suggestive of intense molecular trafficking. Moreover, the roundish nucleoli of control animals change in more irregular nucleoli with numerous small fibrillar centres and abundant dense fibrillar component in GM-fed mice, modifications typical of increased metabolic rate. Accordingly, nucleoplasmic (snRNPs and SC-35) and nucleolar (fibrillarin) splicing factors are more abundant in hepatocyte nuclei of GM-fed than in control mice. In conclusion, our data suggest that GM soybean intake can influence hepatocyte nuclear features in young and adult mice; however, the mechanisms responsible for such alterations remain unknown.

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Available from: Stefano Gavaudan, May 21, 2015
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    • "On the contrary, the majority of experiments conducted with deviations from the standard requirements on the safety assessment of GMO [11] [12] [13], has shown exactly the opposite results. Well-known examples of such publications are articles of [14] [15] [16] [17] [18] [19] [20] and so on. Nevertheless , the debate of this issue is still on, so the completion of science-based evidence of GMO safety has not lost its relevance, particularly, in the field of the next generations' health. "
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    Toxicology Reports 12/2014; 1. DOI:10.1016/j.toxrep.2014.05.013
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    • "Since LDH1 is known to be involved in cell metabolism by favouring the reaction of lactate to pyruvate (Van Hall, 2000), our results could indicate a general increase in cell metabolism. This hypothesis is in agreement not only with results previously obtained in rabbits (Tudisco et al., 2006b) but also with those who found significant modifications of some nuclear features in GM-fed mice suggesting a high metabolic rate and intense molecular trafficking (Malatesta et al., 2002a), signs of hepatorenal toxicity in rat fed transgenic corn MON863 (Seralini et al., 2007), changes in nucleic transcriptional activity, such as chromatin-associated elements in the cell nuclei of liver and pancreas of mice fed diets containing soybean GM for CP4 EPSPS (Malatesta et al., 2002b, 2003, 2005; Vecchio et al., 2004), enlarged spleen and possible impaired spleen function in GM soybean fed Atlantic salmon (Hemre et al., 2005), as well as other several researchers. "
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    ABSTRACT: Using the in vitro gas production technique - which evaluate nutritive value of ruminants feeds - the fermentation kinetics of genetically modified (GM) corn and the gas production of GM soybean were respectively faster and lower compared to their respective conventional counterpart, although the chemical composition were not affect by the genetic modification. Concerning the fate of vegetable DNA in animal organism, which is one of the most important concerns about the use of GM, transgenic DNA sequences were detected in blood and milk from goats fed transgenic soybean as well as from organs of their offspring fed only mother's milk. Considering the potential risks of GM impact, further researches need, including studies to determine DNA transport or entry mechanisms/processes across the epithelial layer of the gastro-intestinal tract into the bloodstream. In any case, the traceability of products from animals fed on genetically modified organisms (GMOs) is crucial.
    World Review of Science Technology and Sustainable Development 01/2014; 11(1):61 - 70. DOI:10.1504/WRSTSD.2014.062383
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    • "Non-adherence to standard procedures makes data interpretation difficult as it is not clear which of the multiple variables that differed between the groups were causative of the observed differences. Research published between 2002 and 2005 by researchers in Italy indicating ultrastructural changes in organs in the liver, pancreas and testes of mice fed diets supplemented with GE and non-GE soya [36,37] has likewise been criticized by independent scientific groups [98,99] regarding a lack of information concerning the source of the GE soybean, the appropriateness of the control soybean used in the diet, and the nutritional composition of the diet. "
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