An integrated microfluidic device for influenza and other genetic analyses. Lab Chip 5:1024-1032

Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
Lab on a Chip (Impact Factor: 5.75). 11/2005; 5(10):1024-32. DOI: 10.1039/b505994a
Source: PubMed

ABSTRACT An integrated microfluidic device capable of performing a variety of genetic assays has been developed as a step towards building systems for widespread dissemination. The device integrates fluidic and thermal components such as heaters, temperature sensors, and addressable valves to control two nanoliter reactors in series followed by an electrophoretic separation. This combination of components is suitable for a variety of genetic analyses. As an example, we have successfully identified sequence-specific hemagglutinin A subtype for the A/LA/1/87 strain of influenza virus. The device uses a compact design and mass production technologies, making it an attractive platform for a variety of widely disseminated applications.

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Available from: Rohit Pal, Mar 25, 2015
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    • "Stationary systems generally allow a small reaction volume and simple system configuration. Detection of micro-organisms in pL scale chamber volumes with chip configurations of up to 1176 parallel reaction chambers have been reported (Marcus et al. , 2006b, Ottesen et al. , 2006, Pal et al. , 2005). Precise sample handling and processing, in addition to ensuring temperature uniformity between chambers, in the setting of increasing numbers of them still pose challenges. "
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    Biotechnology advances 06/2011; 29(6):830-9. DOI:10.1016/j.biotechadv.2011.06.017 · 8.91 Impact Factor
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    • "Implementation of pneumatic pumps and valves has enabled multi-step and highthroughput applications in which massively parallel operations can be performed on a single chip. Examples include the synthesis of radiolabeled imaging probes [2], Sanger sequencing of DNA [1], integrated genetic assays [3] and high-throughput sorting for drug screening [4]. Generally, a highly dense microfluidic chip with several valves and pumps requires many pneumatic connections (tubing) to an external pneumatic controller. "
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    Sensors and Actuators B Chemical 01/2011; DOI:10.1016/j.snb.2010.07.012 · 4.29 Impact Factor
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    • "Another approach is to use microvalves or solid lids to seal the reaction chamber (Moerman et al. 2005; Cheng et al. 2005; Cho et al. 2006; Oh et al. 2005; Pal et al. 2005; Pilarski et al. 2005; Prakash et al. 2006; Toriello et al. 2006, 2008). It is common knowledge that the evaporation rate reduces with increase of the gas pressure around a liquid. "
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