An integrated microfluidic device for influenza and other genetic analyses

Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
Lab on a Chip (Impact Factor: 6.12). 11/2005; 5(10):1024-32. DOI: 10.1039/b505994a
Source: PubMed


An integrated microfluidic device capable of performing a variety of genetic assays has been developed as a step towards building systems for widespread dissemination. The device integrates fluidic and thermal components such as heaters, temperature sensors, and addressable valves to control two nanoliter reactors in series followed by an electrophoretic separation. This combination of components is suitable for a variety of genetic analyses. As an example, we have successfully identified sequence-specific hemagglutinin A subtype for the A/LA/1/87 strain of influenza virus. The device uses a compact design and mass production technologies, making it an attractive platform for a variety of widely disseminated applications.

Download full-text


Available from: Rohit Pal, Mar 25, 2015
  • Source
    • "This concept has been combined with microfluidic cell lysis [80], capillary electrophoresis to confirm PCR products [78,81], or fluorescence microscopy for real-time monitoring of PCR products [82]. Liquid flows through a microchannel continuously, or as discrete liquid plugs within a microchannel. "
    [Show abstract] [Hide abstract]
    ABSTRACT: There have been a number of cases of foodborne illness among humans that are caused by pathogens such as Escherichia coli O157:H7, Salmonella typhimurium, etc. The current practices to detect such pathogenic agents are cell culturing, immunoassays, or polymerase chain reactions (PCRs). These methods are essentially laboratory-based methods that are not at all real-time and thus unavailable for early-monitoring of such pathogens. They are also very difficult to implement in the field. Lab-on-a-chip biosensors, however, have a strong potential to be used in the field since they can be miniaturized and automated; they are also potentially fast and very sensitive. These lab-on-a-chip biosensors can detect pathogens in farms, packaging/processing facilities, delivery/distribution systems, and at the consumer level. There are still several issues to be resolved before applying these lab-on-a-chip sensors to field applications, including the pre-treatment of a sample, proper storage of reagents, full integration into a battery-powered system, and demonstration of very high sensitivity, which are addressed in this review article. Several different types of lab-on-a-chip biosensors, including immunoassay- and PCR-based, have been developed and tested for detecting foodborne pathogens. Their assay performance, including detection limit and assay time, are also summarized. Finally, the use of optical fibers or optical waveguide is discussed as a means to improve the portability and sensitivity of lab-on-a-chip pathogen sensors.
    Sensors 12/2012; 12(8):10713-41. DOI:10.3390/s120810713 · 2.25 Impact Factor
  • Source
    • "Stationary systems generally allow a small reaction volume and simple system configuration. Detection of micro-organisms in pL scale chamber volumes with chip configurations of up to 1176 parallel reaction chambers have been reported (Marcus et al. , 2006b, Ottesen et al. , 2006, Pal et al. , 2005). Precise sample handling and processing, in addition to ensuring temperature uniformity between chambers, in the setting of increasing numbers of them still pose challenges. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Global burdens from existing or emerging infectious diseases emphasize the need for point-of-care (POC) diagnostics to enhance timely recognition and intervention. Molecular approaches based on PCR methods have made significant inroads by improving detection time and accuracy but are still largely hampered by resource-intensive processing in centralized laboratories, thereby precluding their routine bedside- or field-use. Microfluidic technologies have enabled miniaturization of PCR processes onto a chip device with potential benefits including speed, cost, portability, throughput, and automation. In this review, we provide an overview of recent advances in microfluidic PCR technologies and discuss practical issues and perspectives related to implementing them into infectious disease diagnostics.
    Biotechnology advances 06/2011; 29(6):830-9. DOI:10.1016/j.biotechadv.2011.06.017 · 9.02 Impact Factor
  • Source
    • "Implementation of pneumatic pumps and valves has enabled multi-step and highthroughput applications in which massively parallel operations can be performed on a single chip. Examples include the synthesis of radiolabeled imaging probes [2], Sanger sequencing of DNA [1], integrated genetic assays [3] and high-throughput sorting for drug screening [4]. Generally, a highly dense microfluidic chip with several valves and pumps requires many pneumatic connections (tubing) to an external pneumatic controller. "
    [Show abstract] [Hide abstract]
    ABSTRACT: The integration and operation of a large number of components is needed to enable ever more complex and integrated chemical and biological processes on a single microfluidic chip. The capabilities of these chips are often limited by the maximum number of pumps and valves that can be controlled on a single chip, a limitation typically set by the number of pneumatic interconnects available from ancillary hardware. Here, we report a multiplexing approach that greatly reduces the number of external pneumatic connections needed for the operation of a large number of peristaltic pumps. The utility of the approach is demonstrated with a complex microfluidic network capable of generating and routing liquid droplets in a two-phase flow. We also report a set of design rules for the design and operation of multiplexed peristaltic pumps, based on a study of the effect of the number of valves per pump and the valve-to-valve distance on the performance of peristaltic pumps. The multiplexing approach reported here may find application in a wide range of microfluidic chips for chemical and biological applications, especially those that require the integration of many different operations on a single chip and those that need to perform similar operations massively in parallel, in sub-nanoliter volumes.
    Sensors and Actuators B Chemical 01/2011; 151(2-151):384-393. DOI:10.1016/j.snb.2010.07.012 · 4.10 Impact Factor
Show more