Article

Antifungal activity of Lavandula angustifolia essential oil against Candida albicans yeast and mycelial form.

Department of Public Health, University La Sapienza, Rome, Italy.
Medical Mycology (Impact Factor: 2.26). 09/2005; 43(5):391-6. DOI: 10.1080/13693780400004810
Source: PubMed

ABSTRACT The antifungal activity of the essential oil of Lavandula angustifolia Mill. (lavender oil) and its main components, linalool and linalyl acetate, was investigated against 50 clinical isolates of Candida albicans (28 oropharyngeal strains, 22 vaginal strains) and C. albicans ATCC 3153. Growth inhibition, killing time and inhibition of germ tube formation were evaluated. The chemical composition of the essential oil was determined by gas chromatography and mass spectrometry. Lavender oil inhibited C. albicans growth: mean minimum inhibitory concentration (MIC) of 0.69% (vol./vol.) (vaginal strains) and 1.04% (oropharyngeal strains); mean MFC of 1.1% (vaginal strains) and 1.8% (oropharyngeal strains). Linalool was more effective than essential oil: mean MIC of 0.09% (vaginal strains) and 0.29% (oropharyngeal strains); mean MFC of 0.1% (vaginal strains) and 0.3% (oropharyngeal strains). Linalyl acetate was almost ineffective. Lavender oil (2%) killed 100% of the C. albicans ATCC 3153 cells within 15 min; linalool (0.5%) killed 100% of the cells within 30 s. The essential oil inhibited germ tube formation (mean MIC of 0.09%), as did the main components (MIC of 0.11% for linalool and 0.08% for linalyl acetate). Both the essential oil and its main components inhibited hyphal elongation of C. albicans ATCC 3153 (about 50% inhibition at 0.016% with each substance). Lavender oil shows both fungistatic and fungicidal activity against C. albicans strains. At lower concentrations, it inhibits germ tube formation and hyphal elongation, indicating that it is effective against C. albicans dimorphism and may thus reduce fungal progression and the spread of infection in host tissues.

Full-text

Available from: Felicia Diodata D'Auria, Feb 10, 2015
2 Followers
 · 
205 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: INTRODUCTION: Genus Lavandula (Lamiaceae), consist of about 39 species, dozens of subspecies, hundreds of hybrids and cultivars those are widely distributed in the archipelagoes of the Atlantic Ocean and Mediterranean region, it is divided into four main categories; L. angustifolia, L. dentata, L. latifolia and L. intermedia (1 and 2), in Egypt, there are two Lavandula species; L. dentata L. and L. angustifolia Mill. (3); L. dentata commonly known as French lavender is a large plant with greenish-grey foliage late blooming and with characteristic very strong odour (4) while L. angustifolia commonly known as English lavender is a frost hardy species that has many cultivars, habitats and blossom colours (5). The advantageous value of Lavandula species can be referred to the virtue of its versatile therapeutic potentials those can be attributed to the high content of their uniquely constituted volatile oil as well as phenolic content (1, 2 and 6); several researches reported isolation of over 150 compounds from the volatile oil of L. dentata (7) while that of L. angustifolia contained at least 38 different compounds, the chemical compositions of these volatile oils are complex and variable as reported in several investigations for cultivated species in Saudi Arabia, Spain, Morocco, Algeria and Canada (4, 8-20), table, 1. The most prominent chemical constituents in the volatile oil L. dentata (8) are1,8-cineole, α-pinene, β-pinene and p-cymene which exhibit antifungal and antibacterial activities (21 and 8), α-terpineol, terpenen-4-ol and camphene have anti-lice activity (22), whereas those in the volatile oil of L. angustifolia (7 and 23) are linalyl acetate and linalool which have sedative (24 and 25) and local anesthetic effects (26); linalool also exhibited antibacterial (5 and 27-29), fungistatic (21, 27 and 30-32) and insecticidal (22) effects. ABSTRACT Phytochemical studies of two Lavandula species namely L. dentata L. and L. angustifolia Mill. (Lamiaceae) cultivated in Egypt was carried out to subsist positive diagnostic indices for the research of their monographs as well as GC/MS analysis of their volatile oils and quantitative estimation of total phenol and flavonoid contents. The gained results revealed both qualitative and quantitative variation in the chemical composition of the investigated species where twenty-six and thirty-one compounds were identified in the volatile oils of L. dentata L. and L. angustifolia Mill. representing 96.43 % and 98.56 % respectively, the major chemical constituents in the volatile oil of L. dentata were menthe-1,5-dien-8-ol (26.80%), caryophyllene oxide (16.40%) and guiaol (15.36%), while in L. angustifolia were linalyl acetate (18.99%), citronellol (17.36%) and menthe-1,5-dien-8-ol (16.21%), moreover, the calculated values of total phenol and flavonoid contents were 188.50±2.07 mg GAE g -1 , 90.40±1.57 mg QE g -1 , 194.95±2.55 mg GAE g -1 , 98.83±2.68 mg QE g -1 and 167.10±2.30 mg GAE g -1 , 116.15±1.91QE g -1 ,152.50±2.21mg GAE g -1 , 119.95±1.87 mg QE g -1 for their aqueous and methanol extracts respectively. Investigation of their antioxidants impact against Streptozotocin-induced oxidative stress in liver and kidney tissues of albino rats revealed that the methanol extract of L. angustifolia exhibited the highest antioxidant potential followed by, that of L. dentata and volatile oil L. angustifolia in dose dependent manner respectively which afford useful results for developing new natural antioxidant agents.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Background: Silver sulfadiazine (SSD) is the most used topical agent for the treatment of burn wounds. However, it has some side effects such as delayed and incomplete epithelialization, generation of black scars, and limited penetration to the depth of a wound. Objective: The present study investigated the efficacy of herbal combination cream containing Aloe vera gel and essential oils of Lavandula stoechas and Pelargonium roseum in the alleviation of symptoms in patients with superficial second-degree burns and comparison of its effects with those of SSD 1% cream. Methods: One hundred eleven patients with second-degree burns (occurring in the preceding 48 hours and affecting <50% body area) were randomized to receive either herbal cream (n = 56) or SSD 1% cream (n = 55) applied once daily for 14 days. Prevalence of skin dryness and pain severity (assessed using a visual analogue scale) and evidence of infection was determined for patients at baseline as well days 2, 7, and 14. Results: Both groups experienced a significant reduction in the pain severity at day 14 compared to baseline (p <0.001). As for the magnitude of change in pain score, there was a significantly greater reduction from baseline to the seven (p = 0.014) and 14 (p = 0.05) day in the herbal cream compared to control group. The frequency of skin dryness was not significantly different between the groups at any of the assessed time points (p >0.05). There was a single case of infection in the herbal cream group, which cleared with continuation of treatment. Conclusion: Our findings suggested that the herbal cream used here is superior to SSD 1% cream in the alleviation of pain and may serve as a natural alternative for treatment of second-degree burns.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Essential oils compose a heterogenic group of plant products known and used for centuries for cosmetic, disinfectant and therapeutical purposes. Our research considered one of the essential oils, lavender oil, recognized as an antiseptic and insecticide agent also used as an ingredient in bath salts and washing agents for centuries. We studied antibacterial and antifungal activity of pure essential oil as well as commercial cosmetic products: hair shampoo and bath salt, containing lavender oil. It has been shown that concentrations of lavender oil applied in tested cosmetics are too low and do not provide its aseptic activity.