The Zinc-Finger Protein Zat12 Plays a Central Role in Reactive Oxygen and Abiotic Stress Signaling in Arabidopsis

George Mason University, 페어팩스, Virginia, United States
Plant physiology (Impact Factor: 6.84). 11/2005; 139(2):847-56. DOI: 10.1104/pp.105.068254
Source: PubMed


Plant acclimation to environmental stress is controlled by a complex network of regulatory genes that compose distinct stress-response regulons. In contrast to many signaling and regulatory genes that are stress specific, the zinc-finger protein Zat12 responds to a large number of biotic and abiotic stresses. Zat12 is thought to be involved in cold and oxidative stress signaling in Arabidopsis (Arabidopsis thaliana); however, its mode of action and regulation are largely unknown. Using a fusion between the Zat12 promoter and the reporter gene luciferase, we demonstrate that Zat12 expression is activated at the transcriptional level during different abiotic stresses and in response to a wound-induced systemic signal. Using Zat12 gain- and loss-of-function lines, we assign a function for Zat12 during oxidative, osmotic, salinity, high light, and heat stresses. Transcriptional profiling of Zat12-overexpressing plants and wild-type plants subjected to H(2)O(2) stress revealed that constitutive expression of Zat12 in Arabidopsis results in the enhanced expression of oxidative- and light stress-response transcripts. Under specific growth conditions, Zat12 may therefore regulate a collection of transcripts involved in the response of Arabidopsis to high light and oxidative stress. Our results suggest that Zat12 plays a central role in reactive oxygen and abiotic stress signaling in Arabidopsis.

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    • "Root growth, nodule development, and nitrogen-fixation efficiency are particularly affected (Cardovilla et al., 1994). Breeding strategies to improve salt tolerance include exploitation of natural genetic variation and generation of transgenic plants, in which novel genes are introduced or in which the expression levels of existing genes are altered (Austin, 1993; Davletova et al., 2005). Here, we found that plants of all three Medicago species showed higher concentrations of Na + and concomitant decrease in K + compared to controls when subjected to salinity conditions. "
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    ABSTRACT: The present study was conducted to evaluate the responses of three annual Medicago species (M. truncatula, M. laciniata, and M. polymorpha) to salinity. We analyzed publicly available microarray data in NCBI pertaining to salinity-response genes in M. truncatula. Our data search identified Tubby C2 (TLP) and ethylene responsive transcription factor 1 (ERF1) as genes that potentially respond to salinity. We evaluated morpho-physiological traits and the expression of the genes in three Medicago species that had been maintained under control and saline conditions. The analysis of morpho-physiological traits showed that M. polymorpha and M. laciniata were more tolerant to salinity than M. truncatula, as they had lower reductions in biomass and dry root weight and lower increases in anthocyanin concentration. The saline conditions caused a significant increase (P < 0.01) in the expression of TLP in all Medicago species, but caused a significant decrease in the expression of ERF1. Considerable variation in anthocyanin concentrations was found among the three Medicago species. To investigate the cause of this variation, we examined the expression of R2R3MYB, a gene involved in the biosynthesis of anthocyanins. Our analysis showed that saline conditions induced high over-expression of R2R3MYB in all three Medicago spp. The high efficiency of the primer pairs used in qRT-PCR enabled us to compare the expression levels of each gene in the three species. We concluded that the more salt tolerant species showed higher expression of TLP and R2R3MYB under both control and salinity conditions.
    Genetics and molecular research: GMR 09/2015; 14(3):10152-64. DOI:10.4238/2015.August.21.22 · 0.78 Impact Factor
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    • "Subsequently, many C2H2 zinc finger genes have been found in other plants. C2H2 zinc finger proteins participate in many important pathways during different plant growth stages and in stress response (Takatsuji, 1999; Davletova et al., 2005; Huang et al., 2005a,b; Dinneny et al., 2006; Kapoor & Takatsuji, 2006; Li et al., 2006). "
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    ABSTRACT: Arabidopsis trichome formation is an excellent model for studying various aspects of plant cell development and cell differentiation. Our previous works have demonstrated that several C2H2 zinc finger proteins, including GIS, GIS2, ZFP5, ZFP6 and ZFP8, control trichome cell development through GA and cytokinin signalling in Arabidopsis.We identified a novel C2H2 zinc finger protein, GLABROUS INFLORESCENCE STEMS 3 (GIS3), which is a key factor in regulating trichome development in Arabidopsis.In comparison with wild-type plants, loss-of-function of GIS3 mutants exhibited a significantly decreased number of trichomes in cauline leaves, lateral branches, sepals of flowers, and main stems. Overexpression of GIS3 resulted in increased trichome densities in sepal, cauline leaves, lateral branches, main inflorescence stems and in the appearance of ectopic trichomes on carpels.The molecular and genetic analyses show that GIS3 acts upstream of GIS, GIS2, ZFP8 and the key trichome initiation factors, GL1 and GL3. Steroid-inducible gene expression analyses and chromatin immunoprecipitation (ChIP) experiments suggest that GIS and GIS2 are the direct target genes of GIS3.
    New Phytologist 12/2014; 206(1). DOI:10.1111/nph.13218 · 7.67 Impact Factor
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    • "These HSPs are proposed to act as molecular chaperones in protein quality control. Transgenic plants showing a dominant negative variant of heat shock factor 21 suppressed that of Zat12, a water-responsive zinc finger protein required for expression of APX1, and APX1 [167]. Heat shock provokes a rapid reprogramming of gene expression to favor translation of HSPs in which translation factors could play a role. "
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