Canalicular immunostaining of aminopeptidase N (CD13) as a diagnostic marker for hepatocellular carcinoma

Department of Pathology, Otto-von-Guericke-University, Leipziger Str. 44, D-39120 Magdeburg, Germany.
Journal of Clinical Pathology (Impact Factor: 2.92). 11/2005; 58(10):1069-75. DOI: 10.1136/jcp.2005.026328
Source: PubMed


Aminopeptidase N (CD13) is expressed in normal and neoplastic liver tissue, where it exhibits a characteristic canalicular pattern (CD13(can)), similar to that seen for CD10 and when antibodies crossreact with biliary glycoprotein I (p-CEA).
To compare the putative diagnostic use of CD13(can) in differentiating between hepatocellular (HCC) and non-hepatocellular carcinomas metastatic to the liver (non-HCC).
A retrospective study comparing 53 HCC specimens with 32 non-HCC specimens. Immunostaining was performed with HepPar1 and antibodies directed against CD10, CD13, p-CEA, and alpha fetoprotein (AFP).
In the HCC group, a canalicular staining pattern was found for CD13, p-CEA, and CD10 in 51, 43, and 33 specimens, respectively. HepPar1 was positive in 29 and AFP in 17 HCC specimens. In the non-HCC group, canalicular immunostaining for CD10 and p-CEA was confined to non-neoplastic liver tissue. One poorly differentiated cholangiocarcinoma showed apical expression of CD13, resembling to some extent CD13(can). Sensitivity and specificity were 96.2% and 97.0%, respectively, for CD13(can), 81.1% and 100% for p-CEA(can), 62.3% and 100%, for CD10(can), 54.7% and 99.9% for HepPar1, and 32.1% and 100% for AFP.
These results show that CD13(can) is more sensitive in differentiating between HCC and non-HCC than CD10(can), p-CEA(can), HepPar1, and AFP.


Available from: Christoph Röcken, Jan 04, 2014
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    • "For primary staining, monoclonal mouse anti-human APN/CD13 Ab-3 antibody (clone 38C12; Thermo Fisher Scientific, Slangerup, Denmark) was diluted 1 : 40 in TBS buffer with 1% BSA. This antibody has been characterised in several previous studies (Rocken et al, 2005; Terauchi et al, 2007; Mawrin et al, 2010). For secondary staining, we used the anti-mouse EnVision þ System (Dakocytomation, Glostrup, Denmark) with HRP-labelled polymer and DAB solution (Kem-En-Tec, Taastrup, Denmark). "
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