Anti-inflammatory compound resveratrol suppresses homocysteine formation in stimulated human peripheral blood mononuclear cells in vitro.
ABSTRACT Inflammation, immune activation and oxidative stress play a major role in the pathogenesis of cardiovascular disorders. In addition to markers of inflammation, moderate hyperhomocysteinemia is an independent risk factor for cardiovascular disease, and there is a link between the activation of immunocompetent cells and the enhanced formation of homocysteine in vitro. Likewise, anti-inflammatory drugs and nutrients rich in antioxidant vitamins are able to reduce cardiovascular risk and to slow down the atherogenic process. Resveratrol, a phenolic antioxidant synthesized in grapes and vegetables and present in wine, has also been supposed to be beneficial for the prevention of cardiovascular events. Apart from its strong antioxidant properties, resveratrol has also been demonstrated to act as an anti-inflammatory agent. In this study the influence of resveratrol on the production of homocysteine by stimulated human peripheral blood mononuclear cells (PBMCs) was investigated. Results were compared to earlier described effects of the anti-inflammatory compounds aspirin and salicylic acid and of the lipid-lowering drug atorvastatin. Stimulation of PBMCs with the mitogens concanavalin A and phytohemagglutinin induced significantly higher homocysteine accumulation in supernatants compared with unstimulated cells. Treatment with 10-100 muM resveratrol suppressed homocysteine formation in a dose-dependent manner. Resveratrol did not influence the release of homocysteine from resting PBMCs. The data suggest that resveratrol may prevent homocysteine accumulation in the blood by suppressing immune activation cascades and the proliferation of mitogen-driven T-cells. The effect of resveratrol to down-regulate the release of homo-cysteine was comparable to the decline of neopterin concentrations in the same experiments. The suppressive effect of resveratrol was very similar to results obtained earlier with aspirin, salicylic acid and atorvastatin; however, it appeared that doses of compounds needed to reduce homocysteine levels to 50% of stimulated cells were always slightly lower than those necessary to achieve the same effect on neopterin concentrations. The influence of resveratrol and of all the other compounds on homocysteine production appears to be independent of any direct effect on homocysteine biochemistry.
Value in Health 11/2008; 11(6). DOI:10.1016/S1098-3015(10)66448-0 · 2.89 Impact Factor
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ABSTRACT: Tilia species have been used in Asia, Europe and in America to treat anxiety and also for the treatment of colds and in-flammation. The oxygen reactive species (ROS) (hydrogen peroxide (H 2 O 2) and the superoxide anion (O 2) are involve in the balance cell proliferation/death in lymphocytes. It was reported the presence of flavonoids in Tilia species which possess antioxidant properties. The aim of this study was to determine comparatively the effect of an aqueous (AE) and ethanol (E) extract from Tilia x viridis, on the proliferation of tumoral and normal concanavalin A stimulated murine lymphocytes in relation to antioxidant activities such as peroxidase (Px), catalase (CAT) and superoxide dismutase (SOD) activities involves in H 2 O 2 modulation. Also a phytochemical pattern of the two extracts in relation to flavonoids content was determined. Both extracts presented antiproliferative action on both type of lymphocytes but E was more selective on the tumoral lymphocytes inhibition (EC 50 (µg/ml, Mean ±SEM) (tumoral): 50 ± 4; EC 50 ; (normal lympho-cytes): 323 ± 20); this action was related to a high polyphenols content (150 ± 10 mg/g extract) and high "per se" SOD and low Px activities. In conclusion, the extracts could be a source of antioxidant compounds which contribute to a se-lective antiproliferative action on tumoral cells, acting through the modulation of H 2 O 2 levels.
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ABSTRACT: Interest in the potential health beneﬁts of resveratrol, a constituent of red wine, has increased signiﬁcantly in the past decade. Extensive research has been done on it in attempt to reveal its clinical potential both in terms of disease prevention and treatment. Since initially resveratrol has been regarded as an explanation for the French paradox, attention of health professionals and journalists has been drawn to it. Called “promising molecule”, “star molecule” or “wonder drug”, resveratrol has been reposed hope as a cardioprotective, neuroprotective, anticancer, antiinﬂammatory, and antiaging molecule. Here we present state-of-the-resveratrol elucidating its role in promotion of health and prevention of disease.02/2007; Biomed Rev 2007; 18: 89-101(18):89-101. DOI:10.14748/bmr.v18.73