Article

Hepatocyte growth factor as well as vascular endothelial growth factor gene induction effectively promotes liver regeneration after hepatectomy in Solt-Farber rats.

Department of Surgery and Surgical Basic Science, Kyoto University School of Medicine, Kyoto Japan.
Hepato-gastroenterology (impact factor: 0.66). 52(65):1393-7. pp.1393-7
Source: PubMed

ABSTRACT Hepatic oval cells play an important role in liver regeneration when proliferation of mature hepatocytes is inhibited. The aim of this study was to examine the effect of hepatocyte growth factor (HGF), or vascular endothelial growth factor (VEGF) on proliferation of oval cells in the Solt-Farber rat model.
One hour after 70% partial hepatectomy, 2-acetyl-aminofluorene-induced damaged rats were infected intravenously with recombinant adenoviral vectors, encoding rat HGF or human VEGF, or Escherichia coli beta-galactosidase as a control.
The plasma HGF concentrations in the HGF-transferred rats were elevated compared with the other groups at 4 and 7 days after hepatectomy. Oval cells were confirmed by positive staining of both cytokeratin-19 and alpha-fetoprotein. Oval cells around the portal tracts in the HGF or VEGF-transferred rats increased in number compared with the control rats at 7 and 9 days after hepatectomy. The proliferating cell nuclear antigen labeling indices of oval cells and the hepatic regeneration rate after hepatectomy were significantly augmented by the HGF or VEGF treatment. Moreover, cyclin E expression was elevated in the HGF-treated rats.
In the Solt-Farber rat model, HGF or VEGF gene injection effectively promoted liver regeneration after hepatectomy mainly with increased proliferation of hepatic oval cells.

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    Journal of Biomedicine and Biotechnology 01/2010; 2010:984248. · 2.44 Impact Factor
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

7 days
 
70% partial hepatectomy
 
9 days
 
control rats
 
encoding rat HGF
 
Escherichia coli beta-galactosidase
 
Hepatic oval cells
 
hepatic regeneration rate
 
hepatocyte growth factor
 
HGF-transferred rats
 
HGF-treated rats
 
liver regeneration
 
oval cells
 
plasma HGF concentrations
 
portal tracts
 
recombinant adenoviral vectors
 
vascular endothelial growth factor
 
VEGF gene injection
 
VEGF treatment
 
VEGF-transferred rats