Article

[Identification of trehalose-phosphate phosphatase associated with drug-resistance from culture supernatants of isoniazid-resistant Mycobacterium tuberculosis].

State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Llife Science, Fudan University, Shanghai, China.
Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases 11/2004; 27(10):687-9.
Source: PubMed

ABSTRACT To isolate and identify a new protein involved in drug-resistance from culture supernatants of isoniazid (INH)-resistant Mycobacterium tuberculosis.
Bovine albumin of Middlebrook 7H10 culture supernatants of INH-resistant Mycobacterium tuberculosis was removed using diethylamine ethylcellulose affinitive gel chromatography. A specific protein from culture supernatants of three INH-resistant Mycobacterium tuberculosis isolates was separated reproducibly by high performance liquid chromatography and sodium dodecyl sulfate-polyacrylamide electrophoresis, and identified by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-MS).
The protein was trehalose-phosphate phosphatase involved in the biosynthesis of trehalose of cell wall, molecular a eight 46000, isoelectric point 5.3. It could be detected repeatedly from supernatants of three INH resistant Mycobacterium tuberculosis isolates.
Trehalose-phosphate phosphatase represents an excellent potential target site for chemotherapy against Mycobacterium tuberculosis and a new marker in the detection of drug resistance of Mycobacterium tuberculosis.

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Keywords

Bovine albumin
 
culture supernatants
 
diethylamine ethylcellulose affinitive gel chromatography
 
drug resistance
 
drug-resistance
 
excellent potential target site
 
INH resistant Mycobacterium tuberculosis
 
INH)-resistant Mycobacterium tuberculosis
 
INH-resistant Mycobacterium tuberculosis
 
isoelectric point 5.3
 
isoniazid
 
laser desorption ionization time
 
Middlebrook 7H10 culture supernatants
 
Mycobacterium tuberculosis
 
new marker
 
performance liquid chromatography
 
sodium dodecyl sulfate-polyacrylamide electrophoresis
 
trehalose
 
Trehalose-phosphate phosphatase
 

Jun Yue