Article

Low-volume amplification on chemically structured chips using the PowerPlex16 DNA amplification kit

University of Freiburg, Freiburg, Baden-Württemberg, Germany
Deutsche Zeitschrift für die Gesamte Gerichtliche Medizin (Impact Factor: 2.6). 02/2006; 120(1):42-8. DOI: 10.1007/s00414-005-0041-2
Source: PubMed

ABSTRACT In forensic DNA analysis, improvement of DNA typing technologies has always been an issue. It has been shown that DNA amplification in low volumes is a suitable way to enhance the sensitivity and efficiency of amplification. In this study, DNA amplification was performed on a flat, chemically structured glass slide in 1-microl reaction volumes from cell line DNA contents between 1,000 and 4 pg. On-chip DNA amplification reproducibly yielded full allelic profiles from as little as 32 pg of template DNA. Applicability on the simultaneous amplification of 15 short tandem repeats and of a segment of the Amelogenin gene, which are routinely used in forensic DNA analysis, is shown. The results are compared to conventional in-tube amplification carried out in 25-microl reaction volumes.

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