Blueberry flavonoids inhibit matrix metalloproteinase activity in DU145 human prostate cancer cells

Department of Biology, University of Prince Edward Island, 550 UniversityAve., Charlottetown, PE C1A 4P3, Canada.
Biochemistry and Cell Biology (Impact Factor: 2.15). 11/2005; 83(5):637-43. DOI: 10.1139/o05-063
Source: PubMed


Regulation of the matrix metalloproteinases (MMPs), the major mediators of extracellular matrix (ECM) degradation, is crucial to regulate ECM proteolysis, which is important in metastasis. This study examined the effects of 3 flavonoid-enriched fractions (a crude fraction, an anthocyanin-enriched fraction, and a proanthocyanidin-enriched fraction), which were prepared from lowbush blueberries (Vaccinium angustifolium), on MMP activity in DU145 human prostate cancer cells in vitro. Using gelatin gel electrophoresis, MMP activity was evaluated from cells after 24-hr exposure to blueberry fractions. All fractions elicited an ability to decrease the activity of MMP-2 and MMP-9. Of the fractions tested, the proanthocyanidin-enriched fraction was found to be the most effective at inhibiting MMP activity in these cells. No induction of either necrotic or apoptotic cell death was noted in these cells in response to treatment with the blueberry fractions. These findings indicate that flavonoids from blueberry possess the ability to effectively decrease MMP activity, which may decrease overall ECM degradation. This ability may be important in controlling tumor metastasis formation.


Available from: Marva Sweeney, Jul 30, 2015
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    • "Schmidt and colleagues found that blueberry anthocyanins inhibited cell growth of prostate cancer by 11% and inhibited adhesion of Escherichia coli, the bacteria primarily associated with urinary tract infections [58]. Matchett and colleagues discovered that blueberry treatment decreased activity of metastasis mediators MMP-2 and MMP-9 through alteration of protein kinase C (PKC) and mitogen-activated protein (MAP) kinase pathways and increased endogenous tissue inhibitors of metalloproteinases (TIMPs) [92, 93]. "
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    ABSTRACT: Pterostilbene (trans-3,5-dimethoxy-4-hydroxystilbene) is a natural dietary compound and the primary antioxidant component of blueberries. It has increased bioavailability in comparison to other stilbene compounds, which may enhance its dietary benefit and possibly contribute to a valuable clinical effect. Multiple studies have demonstrated the antioxidant activity of pterostilbene in both in vitro and in vivo models illustrating both preventative and therapeutic benefits. The antioxidant activity of pterostilbene has been implicated in anticarcinogenesis, modulation of neurological disease, anti-inflammation, attenuation of vascular disease, and amelioration of diabetes. In this review, we explore the antioxidant properties of pterostilbene and its relationship to common disease pathways and give a summary of the clinical potential of pterostilbene in the prevention and treatment of various medical conditions.
    Oxidative Medicine and Cellular Longevity 04/2013; 2013(15-16):575482. DOI:10.1155/2013/575482 · 3.36 Impact Factor

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    ABSTRACT: Representing the most common flavonoid consumed in the American diet, the flavanols and their polymeric condensation products, the proanthocyanidins, are regarded as functional ingredients in various beverages, whole and processed foods, herbal remedies and supplements. Their presence in food affects food quality parameters including astringency, bitterness, sourness, sweetness, salivary viscosity, aroma, and color formation. The ability of flavonoids to aid food functionality has also been established in terms of microbial stability, foamability, oxidative stability, and heat stability. Phenolic polymer material of the flavonoid family is of considerable interest in the study of red wine, as phenolic quantity and structure can significantly affect wine quality. Although a considerable amount of the phenolic polymer material extracted into red wine during fermentation can be accounted for and characterized as known compounds, a substantial portion of the material remains uncharacterized. During this investigation phenolic polymer material extracted during commercial red wine fermentations (Vitis vinifera Pinot noir) was isolated and analyzed in order to characterize its chemical composition. Phenolic polymer isolates were prepared from samples taken throughout fermentation and isolated by adsorption chromatography. Isolates were subjected to phloroglucinolysis to analyze proanthocyanidin amount as well as subunit composition. Investigation results revealed that known proanthocyanidin content of individual phenolic polymer isolates varied from 27 to 54%. Subsequent analyses were performed in order to quantify material other than known proanthocyanidin subunits. Results of all experiments accounted for up to 82% of the phenolic polymer isolates by mass. Phenolic polymers such as the proanthocyanidins have also become the interest of several investigations due to their potential to provide a variety of health beneficial effects by acting as antioxidant, anticarcinogen, cardiopreventive, antimicrobial, anti-viral, and neuro-protective agents. Such health beneficial effects have been correlated to proanthocyanidin structure-related functionality that allows them to behave as signaling molecules at the molecular level. Consumption of proanthocyanidin rich foods has also reportedly been linked to health detrimental effects including the activation of procarcinogens, reactive oxygen species formation (pro-oxidant activity), hemorrhage formation, hepatotoxicity initiation, pharmacokinetics of therapeutic drug alteration, estrogenic tumor formation, mutagenicity, plasma biochemistry modification, gastroenteritis instigation, antinutritive activity and weight loss. Printout. Thesis (M.S.)--Oregon State University, 2007. Includes bibliographical references (leaves 162-183).
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