Article

Mediated amperometric biosensors for lactic acid based on carbon paste electrodes modified with baker's yeast Saccharomyces cerevisiae.

Institute of Chemistry, Gostauto 9, LT-01108, Vilnius, Lithuania.
Bioelectrochemistry (impact factor: 3.76). 06/2006; 68(2):191-6. DOI:10.1016/j.bioelechem.2005.08.002 pp.191-6
Source: PubMed

ABSTRACT Carbon paste electrodes modified with baker's yeast Saccharomyces cerevisiae (a source of flavocytochrome b(2)) were investigated as amperometric biosensors for lactic acid. Phenazine methosulphate was used as a mediator. The optimal operational conditions of the electrodes were: an operating potential 0.0 V, solution pH 7.2, concentration of phenazine methosulphate in solution 0.2 mM. A linear range in the dependence of the current responses on the concentration of lactic acid was up to 1 mM. The suitability of the electrodes for determination of lactic acid in milk and dairy products such as kefir and yoghurt was tested. The yeast cells in the paste remained viable at least for 1 month.

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Keywords

1 month
 
baker's yeast Saccharomyces cerevisiae
 
Carbon paste electrodes
 
current responses
 
dairy products
 
flavocytochrome b(2)
 
lactic acid
 
linear range
 
operating potential 0.0
 
optimal operational conditions
 
phenazine methosulphate
 
solution pH 7.2