Expressions of nuclear factor KB, inducible nitric oxide synthase and vascular endothelial growth factor in adenoid cystic carcinoma of salivary glands: Correlationswith the angiogenesis and clinical outcome

Key Lab for Oral Biomedical Engineering, Ministry of Education, School of Stomatology, Wuhan University, Wuhan, PR China.
Clinical Cancer Research (Impact Factor: 8.72). 10/2005; 11(20):7334-43. DOI: 10.1158/1078-0432.CCR-05-0241
Source: PubMed


To evaluate the expressions of nuclear factor kappaB (NF-kappaB p65), inducible nitric oxide synthase enzyme (iNOS), and vascular endothelial growth factor (VEGF) in relation to angiogenesis (microvessel density, MVD) and clinical outcomes in adenoid cystic carcinoma (ACC) of salivary glands.
Immunohistochemical staining was used to quantify the protein expression levels of NF-kappaB p65, iNOS, and VEGF in 80 surgically resected ACCs and 20 normal salivary tissues. In all cases of ACCs, MVD was evaluated by counting CD34-reactive endothelial cells or endothelial cell clusters.
The nuclear localization of NF-kappaB p65 was only detected in ACC cells. Both iNOS and VEGF staining activities in ACCs were more significant than those in normal gland tissues (P < 0.01). MVD had significant correlations with NF-kappaB p65, iNOS, and VEGF expressions (P < 0.01). In three histologic types of ACCs, the NF-kappaB, iNOS, VEGF expressions, and MVD were significantly higher in solid type than in cribriform and tubular types (P < 0.01). The NF-kappaB, iNOS, VEGF expressions, and MVD were significantly correlated with clinical stage, tumor size, vascular invasion, recurrence, and metastasis (P < 0.05). Multivariate analysis showed NF-kappaB, iNOS and VEGF expression, MVD, solid histotype, and perineural invasion had an independent prognostic effect on overall survival.
The expressions of NF-kappaB p65, iNOS, and VEGF were related with MVD. Clinical outcomes raised the possibility that the overexpression of these cytokines might contribute to tumor angiogenesis and have prognostic value in ACCs.

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    • "After being mounted in a xylene-based medium (VWR International, Radnor, PA, USA), the sections were examined under a light microscope (Leica DMLB; Leica Biosystems, Nussloch, Germany). Microvessel density (MVD) was evaluated according to the guidelines already published elsewhere [21–23]. Positive staining of a single endothelial cell or cluster of endothelial cells was defined as microvessel. "
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