Vascular responses to extractable fractions of Ilex paraguariensis in rats fed standard and high-cholesterol diets.
ABSTRACT The authors investigated the vasorelaxant properties of the aqueous (Aq-EF) and acid n-butanolic (acn-BuOH) extractable fractions from Ilex paraguariensis leaves. Perfusion pressure was evaluated using isolated and perfused mesenteric arterial beds (MABs) from rats fed hypercholesterolemic and standard diets. Extract-induced vasorelaxation in the presence and absence of various inhibitors was examined following precontraction of the MABs with methoxamine (30 microM) solution. In hypercholesterolemic-diet rats, relaxation in intact MABs was significantly decreased with ac-n-BuOH-EF bolus (300, 600, 900 microg) in comparison to those in standard-diet rats. After the endothelium was stripped from the MABs, the vascular responses to ac-n-BuOH-EF and 900 microg bolus of Aq-EF were significantly changed. Treatment of the MABs with an inhibitor of nitric oxide synthase, N(G)-nitro-L-arginine methylester hydrochloride (L-NAME, 10 mM), did not change either ac-n-BuOH-EF- or Aq-EF-induced vasodilation except for the 900 microg bolus of Aq-EF. The guanilate cyclase inhibitor methylene blue (100 microM) did not affect vasodilation for either fraction in the MABs from the hypercholesterolemic-diet rats. The chronic oral administration of I. paraguariensis extract in hypercholesterolemic-diet rats resulted in a significant reduction in serum levels of cholesterol and triglycerides. These results suggest that I. paraguariensis ac-n-BuOH-EF and Aq-EF induce vasodilation in standard-diet rats in a dose-dependent manner and that the hypercholesterolemic diet substantially reduced the effect of ac-n-BuOH-EF on precontracted MABs.
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- "), lipolytic, hypolipidemic (Kim et al., 2012; Martins et al., 2009; Paganini Stein et al., 2005; Pang et al., 2008), prevention of lipoprotein oxidation (Bracesco et al., 2011), and thermogenic (Martinet et al., 1999) activities. Weight reduction induced by mate had been associated with these activities, but other factors may be involved, such as cholesterol binding by saponins in intestinal lumen (Francis et al., 2002), appetite suppression (Andersen and Fogh, 2001; Pang et al., 2008), and pancreatic lipase inhibition (Kim et al., 2009; Martins et al., 2009; Sugimoto et al., 2009). "
ABSTRACT: Ilex paraguariensis A. St. Hilaire (mate) has traditionally been used in several South American countries to prepare tea-like beverages having stimulant effects on the CNS and appetite. In recent years, however, mate preparations have been recommended putatively as an appetite suppressant and slimming remedy. Moreover, studies carried out on either normal or diet-induced obese rats treated with mate extracts revealed anti-obesity and satiety effects, thus refuting ethnopharmacological data. In this work, the effect of mate on the intra-abdominal and epididymal fat, and glucose oxidation levels after oral administration in male Wistar rats, was studied using crude extract from leaves, unripe fruits, and a chemically well-defined purified saponin fraction (MSF).Journal of ethnopharmacology 10/2012; 144(3). DOI:10.1016/j.jep.2012.10.023 · 2.94 Impact Factor
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- "c o m Nutrition 28 (2012) 1157–1164 potential benefits of I. paraguariensis to human health, particularly in the prevention of cardiovascular diseases . Studies on in vitro systems and in vivo models have shown that yerba mate possesses vasorelaxation   , anti-inflammatory  , anti-atherosclerotic , hypocholesterolemic   , and antioxidant [1–3,13] properties. Recently, our research group demonstrated that the ingestion of green or roasted yerba mate infusions decreased low-density lipoprotein cholesterol and increased high-density lipoprotein cholesterol levels in healthy subjects, particularly in hypercholesterolemic individuals on statin therapy , and in prediabetic or type 2 diabetic subjects . "
ABSTRACT: Paraoxonase-2 (PON-2) is an intracellular antioxidant enzyme that can be modulated by polyphenols. The aim of this study was to verify whether yerba mate (Ilex paraguariensis), a plant species rich in phenolic compounds, modulates gene expression and the activity of PON-2 in macrophages in vitro and in monocytes from peripheral blood and monocyte-derived macrophages obtained after the ingestion of green or roasted yerba mate infusions by healthy subjects. THP-1 macrophages were incubated with increasing amounts of yerba mate extracts or chlorogenic and caffeic acids (1-10 μmol/L). The in vivo effects of yerba mate or water (control) intakes were evaluated acutely (2 h after ingestion) and in the short term (after daily ingestion for 7 d) in 20 healthy women. In general, there was no difference between the two kinds of yerba mate studied. Yerba mate extracts or chlorogenic acid at 1 and 3 μmol/L increased PON-2 relative gene expression in THP-1 macrophages (P < 0.05), whereas higher concentrations (5 and 10 μmol/L) increased the activity only. Caffeic acid induced PON-2 activity only. The acute ingestion of yerba mate infusions increased relative gene expression and PON-2 activity in monocytes (P < 0.05), whereas the consumption of yerba mate for 7 d increased PON-2 relative gene expression (P < 0.05) and had a tendency to increase PON-2 activity in monocytes and monocyte-derived macrophages. It is suggested that green or roasted yerba mate modulates positively the mRNA relative expression and activity of the PON-2 enzyme in monocytes and macrophages, which may prevent cellular oxidative stress.Nutrition 09/2012; 28(11-12):1157-64. DOI:10.1016/j.nut.2012.04.011 · 3.05 Impact Factor
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ABSTRACT: Maté (Ilex paraguariensis St. Hilaire) is a plant originary from the subtropical region of South America, and present in the South of Brazil, North of Argentina, Paraguay and Uruguay. Maté beverages have been widely consumed for hundreds of years as infusions popularly known as chimarrão, tererê (both from green dried mate leaves) and maté tea (roasted mate leaves). The popular medicine and the herbalists recommend it for arthritis, migraines, constipation, rheumatism, hemorrhoids, obesity, fatigue, retention of liquid, hypertension, and for stomach and liver diseases. Recently published research has proved scientifically the actions of maté which may explain many of the cited pharmacologic effects such as its chemopreventive activity, cholerectic effect and intestinal propulsion, vasodilatation effect, inhibition of the glication and as a free radical scavenger. Maté beverages are rich in many bioactive compounds such as caffeine, phenolic compounds (mainly phenolic acids) and saponins. This review discusses the latest scientific data on maté physiological properties and their correlation with the bioactive compounds present in the maté leaves and aqueous infusions.