HIV-infected cells are major inducers of plasmacytoid dendritic cell interferon production, maturation, and migration

Department of Medicine, Division Hematology/Oncology, University of California, San Francisco, CA 94143-0128, USA.
Virology (Impact Factor: 3.28). 01/2006; 343(2):256-66. DOI: 10.1016/j.virol.2005.09.059
Source: PubMed

ABSTRACT Plasmacytoid dendritic cells (PDC), natural type-1 interferon (IFN) producing cells, could play a role in the innate anti-HIV immune response. Previous reports indicated that PDC IFN production is induced by HIV. Our results show a more robust IFN induction when purified PDC (>95%) were exposed to HIV-infected cells. This effect was not observed with non-viable cells, DNA, and RNA extracted from infected cells, and viral proteins. The response was blocked by anti-CD4 and neutralizing anti-gp120 antibodies as well as soluble CD4. IFN induction by HIV-infected cells was also prevented by low-dose chloroquine, which inhibits endosomal acidification. PDC IFN release resulted in reduced HIV production by infected CD4+ cells, supporting an anti-HIV activity of PDC. Stimulated CD4+ cells induced PDC activation and maturation; markers for PDC migration (CCR7) were enhanced by HIV-infected CD4+ cells only. This latter finding could explain the decline in circulating PDC in HIV-infected individuals.

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Available from: Barbara Schmidt, Jun 26, 2015
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