Labeling of fusion proteins of O-alkylguanine-DNA alkyltransferase with small molecules in vivo and in vitro
Institute of Molecular and Biological Chemistry, Ecole Polytechnique Fédérale de Lausanne, Lausanne CH-1015, Switzerland. Methods
(Impact Factor: 3.65).
05/2004; 32(4):437-44. DOI: 10.1016/j.ymeth.2003.10.007
The in vivo and in vitro labeling of fusion proteins with synthetic molecules capable of probing and controlling protein function has the potential to become an important method in functional genomics and proteomics. We have recently introduced an approach for the specific labeling of fusion proteins, which is based on the generation of fusion proteins with the human DNA repair protein O6-alkylguanine-DNA alkyltransferase (hAGT) and the irreversible reaction of hAGT with O6-benzylguanine derivatives. Here, we report optimized protocols for the synthesis of O6-benzylguanine derivatives and the use of such derivatives for the labeling of different hAGT fusion proteins in vivo and in vitro.
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.