Evaluation of the association between null genotypes of glutathione-S-transferases and Behcet's disease.
ABSTRACT Glutathione S-transferases (GST) play an important role in oxidative stress related syndromes. An imbalance of the oxidant and antioxidant systems is important in the pathogenesis of Behcet's disease (BD). The objective of this study was to evaluate the association of null genotypes of GST-M1 and GST-T1 with BD since some preliminary molecular genetic data were recently published. Ninety-four Turkish BD patients (42 male, 52 female, 37.1+/-10.4 years) and 140 healthy volunteers (70 male, 70 female, 36.8+/-11.7 years) matched for age and gender with the patients as the control group were included in the study. Distributions of GST-M1 and GST-T1 genotypes were determined by multiplexed PCR using three sets of primers for GST-M1, GST-T1, and beta-globulin genes. There was no association between BD and the frequencies of GST-M1 and GST-T1 null genotypes when compared to controls by separate analysis. However, by cross and pooled combination analysis there was a significant association between the frequencies of pooled GSTs with one or both null genotypes in BD and controls. This is the first evidence that the association between the frequencies of GST-M1 and GST-T1 null genotypes and BD might be dependent on the interaction of multiple null allele polymorphisms rather than a single null allele of GST-M1 and GST-T1.
SourceAvailable from: Amal Saad-Hussein[Show abstract] [Hide abstract]
ABSTRACT: Little is known whether occupational exposure to aflatoxin might have a potential hazard. The present work aimed to study the hepatotoxic effects of occupational exposure to AFB1 as a metabolite of high Aspergillus concentrations in the working environments, and the polymorphism of GST gene in exposed workers. The study was performed on 97 flour mill workers exposed to high Aspergillus concentrations and 78 non-exposed controls. The levels of AFB1/Alb (ng/g), AST and ALT of the workers were significantly higher than the controls, while there was no significance difference in the ALP levels between the two groups. The present results revealed that AFB1/Alb levels were significantly higher in the workers with the different GST alleles compared to the control groups. Additionally, AFB1/Alb levels were significantly higher in the workers with GSTT1 compared to the workers with different GST alleles (GSTM1 and Null (GSTT1&GSTM1)) and the controls with Null (GSTT1&GSTM1) alleles. In conclusion, occupational exposure to the high concentrations of Aspergillus in the workplace may cause an increase in the AFB1/Alb and the liver enzymes in flour mill workers. These results also showed that the Null (GSTM1 & GSTT1) alleles are the most common type in the studied population. The workers with GSTT1 have lower ability to detoxify AFB1.
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ABSTRACT: Several studies have reported increased oxidation of lipids, proteins and DNA in the brains of patients with Alzheimer disease (AD). Moreover, these patients display differences in the activity and polymorphisms of the genes encoding the enzymes GST (T1, M1) and MnSOD. For these reasons, we designed a study of the variability in GSTT1, GSTM1, and MnSOD genes in healthy and AD groups from a Venezuelan population.Neurologia (Barcelona, Spain) 12/2014; DOI:10.1016/j.nrl.2014.10.012 · 1.35 Impact Factor
Dataset: AFs GST gene millers