NO signaling in ARE-mediated gene expression

College of Pharmacy, Seoul National University, Kwanak-gu, Seoul, South Korea.
Methods in Enzymology (Impact Factor: 2.09). 02/2005; 396:341-9. DOI: 10.1016/S0076-6879(05)96028-X
Source: PubMed


Nitric oxide (NO) and peroxynitrite, which serve as cell signal molecules, activate the antioxidant response element (ARE) for the induction of phase II antioxidant enzymes as an adaptive response. The reactive nitrogen species plays an essential role in Nrf2 activation and Nrf2 binding to the ARE present in the target genes. In this chapter, we describe the system by which the NO signaling pathway regulates ARE-mediated gene expression, which includes immunochemical assessment and gel shift analysis of Nrf2 activation.

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    • "Moreover, peroxynitrite , another reactive nitrogen species, produces the induction of the Nrf2–Keap1 pathway (Kang et al. 2002). Park and Kim (2005) showed that treatment with 3- morpholinosydnonimene hypochloride (SIN-1), a peroxynitrite-induced compound, generates Nrf2 translocation to the nucleus and its coupling to ARE. Similar to NO, this activation protects cells against peroxynitrite-induced apoptosis (Li et al. 2006). "
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