Stress-induced accumulation of glycerol in the flesh fly, Sarcophaga bullata: Evidence indicating anti-desiccant and cryoprotectant functions of this polyol and a role for the brain in coordinating the response

Department of Biology, Wittenberg University, Springfield, OH 45501, USA.
Journal of Insect Physiology (Impact Factor: 2.47). 03/2006; 52(2):202-14. DOI: 10.1016/j.jinsphys.2005.10.005
Source: PubMed


Nondiapausing larvae of the flesh fly, Sarcophaga bullata, responded to several forms of short-term environmental stress (low temperature, anoxia and desiccation) by accumulating glycerol. Elevation of this polyol, regardless of the type of stress that induced accumulation, conferred cold resistance: larvae with high glycerol levels were 3-4 times more tolerant of a 2h exposure to -10 degrees C than unstressed larvae. Protection against low temperature injury, as well as dehydration, was also attained by injection of exogenous glycerol into third instar larvae. This artificially induced cold hardiness was only temporary: when glycerol-injected larvae were exposed to -10 degrees C immediately after injection, survival was high, but none survived if they were injected and then held at 25 degrees C for 2 days before the -10 degrees C exposure. Larvae ligated behind the brain immediately after low temperature exposure failed to accumulate glycerol, but glycerol did accumulate in larvae ligated 6-24h after cold treatment, thus implying a critical role for the brain in initiating glycerol production. Interestingly, a much shorter exposure (2h) to low temperature was sufficient to reduce the maximum rate of water loss. Collectively, these observations suggest that multiple pathways may be exploited in response to stress: one pathway is most likely associated with rapid cold hardening (RCH) which generates immediate protection, and a second pathway remains activated for a longer period to enhance the initial protection afforded by glycerol.

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    • "Although the alterations in these sugars are small, they may have beneficial effects on membrane function (Overgaard et al., 2014). Moreover, Yoder et al. (2006) demonstrated that an increase in the glycerol levels significantly improved chill tolerance, as may also happen for G. coquereliana. Together, our results demonstrate that the tropical cockroach G. coquereliana responds to cold stress by a shift in biochemical pathways , which results in the synthesis of compounds that protect the insect from or desensitize it to temperature changes. "
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    • "Glycerol and sorbitol, as the most common cryoprotectant molecules, lower the supercooling points and protect against protein denaturation 69. Glycerol kinase (CL3214.Contig1) is a rate-limiting enzyme in glycerol utilization 70, increasingly expressed during diapause to promote glycerol biosynthesis which was stress-induced and related to high cold resistance (Figure 8) 71. In a specific way, sorbitol dehydrogenase (CL3305.Contig1), related to diapause termination in eggs of B. mori 72, was upregulated in LD (Figure 8). "
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    International journal of biological sciences 09/2014; 10(9):1051-63. DOI:10.7150/ijbs.9438 · 4.51 Impact Factor
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    • "Cold sensing may be centrally coordinated via brain control to initiate glycerol production (Yoder et al., 2006). Alternatively, calcium influx in all tissues exposed to low temperature may autonomously induce RCH (Teets et al., 2013). "
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