Article

Imaging of gene expression in living cells and tissues.

Albert Einstein College of Medicine, Department of Anatomy and Structural Biology and Biochemistry, Biophotonics Center, 1300 Morris Park Avenue, Bronx, New York 10461, USA.
Journal of Biomedical Optics (Impact Factor: 2.75). 01/2005; 10(5):051406. DOI: 10.1117/1.2103032
Source: PubMed

ABSTRACT It is possible to observe gene expression within single cells using a tetracycline inducible promoter for activation. Transcription can be observed by using a fluorescent fusion protein to bind nascent RNA. Ultimately, it is desirable to activate a reporter gene within a single cell with only photons. This is achieved by preparing a chemically altered transcription factor that is functionally unable to activate a reporter gene until it is exposed to photon excitation. We apply two-photon imaging to visualize tumor cells expressing a transgene and ultimately this approach will provide the means to activate a specific gene within a single cell within any tissue to ultimately observe its functional significance in situ.

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