Capillary electrophoresis of proteins 2003–2005

Alcor BioSeparations, Santa Clara, CA, USA.
Electrophoresis (Impact Factor: 3.03). 01/2006; 27(1):126-41. DOI: 10.1002/elps.200500567
Source: PubMed

ABSTRACT This review article with 304 references describes recent developments in CE of proteins, and covers the two years since the previous review (Hutterer, K., Dolník, V., Electrophoresis 2003, 24, 3998-4012) through Spring 2005. It covers topics related to CE of proteins, including modeling of the electrophoretic migration of proteins, sample pretreatment, wall coatings, improving separation, various forms of detection, special electrophoretic techniques such as affinity CE, CIEF, and applications of CE to the analysis of proteins in real-world samples including human body fluids, food and agricultural samples, protein pharmaceuticals, and recombinant protein preparations.

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Available from: Vlad Dolnik, Jun 27, 2014
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    • "Nowadays, electrophoretic separation methods are widely applied to study polyelectrolytes (PEs) such as proteins, DNA and synthetic polymers [1] [2] [3]. While there exist several theories [4] [5] [6] [7] that have been successfully used to describe qualitatively the experimentally observed behaviour of various PEs, there are still many open problems to address. "
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    ABSTRACT: The effect of hydrodynamic interactions on the free-solution electrophoresis of polyelectrolytes is investigated with coarse-grained molecular dynamics simulations. By comparing the results to simulations with switched-off hydrodynamic interactions, we demonstrate their importance in modelling the experimentally observed behaviour. In order to quantify the hydrodynamic interactions between the polyelectrolyte and the solution, we present a novel way to estimate its effective charge. We obtain an effective friction that is different from the hydrodynamic friction obtained from diffusion measurements. This effective friction is used to explain the constant electrophoretic mobility for longer chains. To further emphasize the importance of hydrodynamic interactions, we apply the model to end-labelled free-solution electrophoresis.
    Journal of Physics Condensed Matter 11/2008; 20(49):494217. DOI:10.1088/0953-8984/20/49/494217 · 2.35 Impact Factor
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    ABSTRACT: Cereal grains are widely used for human food and animal feed throughout the world. Distinction between the various genotypes of any cereal species is important to segregate grains according to utilization type. DNA analysis indicates genotype (variety), whereas protein composition provides information about both variety and likely processing properties, reflecting the contributions of both genotype and growth/storage conditions. Standard methods of protein and DNA analysis involve gel electrophoresis in various formats. Capillary electrophoresis (CE) is one of the newer techniques to be so used. CE is a valuable addition to other methods of cereal protein and DNA analysis and should, in time, be applicable to analyzing DNA and all protein classes from all cereal grains. This review focuses on methods for distinguishing genotypes based on th e protein and DNA composition of the grain by capillary electrophoresis. Microfluidic capillary electrophoresis (Lab-on-a-chip), a newly advanced and rapid technique, is also discussed.
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    ABSTRACT: The coupling of CE with MS detection, a relatively recent hyphenated technique, has gained increasing respect in the field of bioanalytical applications over the past few years. The first part of this review presents CE-MS applications dealing with drug bioanalysis, including forensic analysis and metabolism studies. Practical considerations to achieve a robust and sensitive CE-MS coupling are also presented. It is indeed essential to strictly control some critical electrospray parameters, such as the sheath liquid composition and flow rate, the nebulizing gas pressure as well as the capillary outlet position. The second part of the review critically describes the applications of CE coupled on-line to MS for the identification of biomarkers in body fluids for diagnostic purposes. Since the sample preparation procedures strongly differ according to the intended use (drug bioanalysis or biomarker discovery), they are discussed separately, taking into account the particular properties of plasma and urine matrices.
    Electrophoresis 07/2006; 27(13):2616-29. DOI:10.1002/elps.200500934 · 3.03 Impact Factor
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