Establishment of HPLC-DAD-MS fingerprint of fresh Houttuynia cordata.

School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Special Administrative Region, PR China.
CHEMICAL & PHARMACEUTICAL BULLETIN (Impact Factor: 1.56). 01/2006; 53(12):1604-9. DOI:10.1248/cpb.53.1604
Source: PubMed

ABSTRACT A HPLC-DAD-MS fingerprint method of fresh Houttuynia cordata THUNB. was developed basing on the consistent chromatographic features among 11 batches of authentic samples. Major chemical components including phenolic compounds, flavones and alkaloids were simultaneously analyzed. Eleven common peaks in the fingerprint were chosen and identified by comparing their UV and ESI-MS data with the authentic compounds. The unique properties of this HPLC-DAD-MS fingerprint were successfully applied to analyze and differentiate samples from different geographical origins, processing methods and various medicinal parts of H. cordata. The results showed that these variations will give rise to differences in identities and/or abundance of chemical compounds, indicating that a comprehensive quality evaluation of those major ingredients in H. cordata is critical to assess and represent its overall quality.

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    ABSTRACT: This study is designed to compare the antioxidant potential of a water-soluble polysaccharide (HCP) with solvent extracts (water, ethanol, ethyl acetate and chloroform) from Houttuynia cordata Thunb. The results showed that polar water extract exhibited the highest reducing power and scavenging activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, superoxide radical and hydroxyl radical, which were correlated with its high level of biopolymer HCP. Furthermore, the active HCP was identified as an acid hetero-polysaccharide by a rapid HPLC technology within 20 min, and galacturonic acid (29.4%) and galactose (24.0%) were approved as the prominent components of HCP, followed by rhamnose (17.2%), arabinose (13.5%), glucuronic acid (6.8%), glucose (5.3%), xylose (2.1%) and mannose (1.8%) in the molar percentages. This finding suggests that HCP is one of the main active ingredients responsible for antioxidant effect of H. cordata, which might be valuable as a natural antioxidant source applied in both healthy medicine and food industry.
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    ABSTRACT: The present review is an attempt to put an insight into a medicinal plant Houttuynia cordata Thunb, which is indigenous to North-East India and China. It is an aromatic medicinal herb belonging to family Saururaceae and is restricted to specialized moist habitats. The review provides detailed information regarding the morphology, distribution, phytochemistry, ethnopharmacological uses and also describes various pharmacological activities reported on the plant H. cordata. The review describes therapeutic efficacy of the whole plant and its extracts, fractions and isolated compounds in different diseased condition. Among the important pharmacological activities reported includes, anti-mutagenic, anti-cancer, adjuvanticity, anti-obesity, hepatoprotective, anti-viral, anti-bacterial, anti-inflammatory, free radical scavenging, anti-microbial, anti-allergic, anti-leukemic, chronic sinusitis and nasal polyps activities. Thus, the present review will act as a source of referential information to researchers to perform clinical studies on isolated compounds that may serve the society and will help in improving human health care system.
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    ABSTRACT: A simple, sensitive, and accurate HPLC-CL method was developed for the first time to determine 2-undecanone in the dried aerial parts of Houttuynia cordata Thunb (HC), which has been widely used as one of the more common traditional Chinese medicines (TCMs). This HPLC-CL assay was performed on a reversed-phase C18 column (250 mm × 4.6 mm, 5 µm) at the column temperature of 35°C. The mobile phase was composed of acetonitrile and water (50:50, v/v). The flow rate was 1.0 mL/min. The chemiluminescence was produced by luminol oxidation during a reactor of which was based on the analytes enhancement effect on the CL reaction between luminol and K3Fe(CN)6. The calibration curves showed good linear regression (r = 0.9993) within the test range of 3.0 × 10−1.0 × 10 g/mL. The established method showed good precision with average intra-day and inter-day variations of 2.08% and 1.85%, respectively, and a recovery of 99.55% for the 2-undecanone. The validated method was successfully applied for the determination of 2-undecanone in 9 samples from 9 different cultivation regions. The present HPLC–CL method was demonstrated to be very helpful in the quantification of 2-undecanone in HC.
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