Establishment of HPLC-DAD-MS fingerprint of fresh Houttuynia cordata.

School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Special Administrative Region, PR China.
CHEMICAL & PHARMACEUTICAL BULLETIN (Impact Factor: 1.38). 01/2006; 53(12):1604-9. DOI: 10.1248/cpb.53.1604
Source: PubMed

ABSTRACT A HPLC-DAD-MS fingerprint method of fresh Houttuynia cordata THUNB. was developed basing on the consistent chromatographic features among 11 batches of authentic samples. Major chemical components including phenolic compounds, flavones and alkaloids were simultaneously analyzed. Eleven common peaks in the fingerprint were chosen and identified by comparing their UV and ESI-MS data with the authentic compounds. The unique properties of this HPLC-DAD-MS fingerprint were successfully applied to analyze and differentiate samples from different geographical origins, processing methods and various medicinal parts of H. cordata. The results showed that these variations will give rise to differences in identities and/or abundance of chemical compounds, indicating that a comprehensive quality evaluation of those major ingredients in H. cordata is critical to assess and represent its overall quality.

  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Herpes simplex virus (HSV), a common latent virus in humans, causes certain severe diseases. Extensive use of acyclovir (ACV) results in the development of drug-resistant HSV strains, hence, there is an urgent need to develop new drugs to treat HSV infection. Houttuynia cordata (H. cordata), a natural herbal medicine, has been reported to exhibit anti-HSV effects which is partly NF-κB-dependent. However, the molecular mechanisms by which H. cordata inhibits HSV infection are not elucidated thoroughly. Here, we report that H. cordata water extracts (HCWEs) inhibit the infection of HSV-1, HSV-2, and acyclovir-resistant HSV-1 mainly via blocking viral binding and penetration in the beginning of infection. HCWEs also suppress HSV replication. Furthermore, HCWEs attenuate the first-wave of NF-κB activation, which is essential for viral gene expressions. Further analysis of six compounds in HCWEs revealed that quercetin and isoquercitrin inhibit NF-κB activation and additionally, quercetin also has an inhibitory effect on viral entry. These results indicate that HCWEs can inhibit HSV infection through multiple mechanisms and could be a potential lead for development of new drugs for treating HSV.
    PLoS ONE 02/2015; 10(2):e0115475. DOI:10.1371/journal.pone.0115475 · 3.53 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Objectives Ciclosporin and sirolimus, two immunosuppressive agents with narrow therapeutic windows, are mainly metabolized by Cytochrome 3A4 (CYP3A4). A clinical case of toxic blood levels of these drugs after the consumption of a ‘24-flavours’ tea was reported. This study aims to identify the causative ingredients of the 24-flavour herbal tea in the inhibition of CYP3A4 metabolism.Methods Two commercially available 24-flavour tea products purchased in Hong Kong and the six plant constituents were tested for their CYP3A4 inhibitory effects utilizing an in-vitro fluorometric assay.Key findingsOf the commercially available teas available in Hong Kong, the most potent inhibitory effect was observed with the tea consumed in the initial clinical case. Of the six universal constituents, chrysanthemum exhibited the greatest inhibitory effect, with an IC50 of 95.7 μg/ml. Dandelion, liquorice and bishop's weed have IC50 of 140.6, 148.4 and 185.5 μg/ml, respectively. Field mint and Japanese honeysuckle have weaker inhibitory effect on CYP3A4 with IC50 of 1153.3 and 1466.3 μg/ml.Conclusions This study confirms the possible implication of herbal tea constituents in the inhibition of ciclosporin and sirolimus’ CYP3A4 metabolism. Combined usage of herbal teas with drug should be closely monitored.
    06/2014; 66(10). DOI:10.1111/jphp.12270
  • [Show abstract] [Hide abstract]
    ABSTRACT: A novel method that combines high performance liquid chromatography (HPLC) fingerprint with blending technology was developed and validated for ensuring quality stability of Zhou's prescription extract. For fingerprint analysis, 32 peaks were selected as the common peaks by comparing the chromatograms of 15 batches of Zhou's prescription extracts. Meanwhile, these common peaks were identified by using electronic spray ion mass spectrometry. The batch, which contained herbs that were obtained from geo-authentic habitats during their best harvest times, was selected as the standard extract (SE). The quality difference between SE and 14 batches of Zhou's prescription extracts was evaluated by using the fingerprint similarity, relative deviation of content (RDC), hierarchical clustering analysis (HCA) and principal component analysis (PCA), and narrowed by applying blending technology that could lead to homogenization of high quality of the 14 batches of Zhou's prescription extracts by using nonlinear programming. According to the mode of finding difference and narrowing difference, the previous two kinds of quality control method was combined smartly to ensure the quality stability of Zhou's prescription. Finally, this method was verified and the results indicated that fingerprint similarities of 10 different blending schemes increased to the range of 0.9208 to 0.9797 from 0.7338 to 0.8925. The average RDCs of 28 index components decreased to the range of 0.1549 to 0.2790 from 0.4768 to 0.6083. The diagrams of HCA and PCA show that the 10 blending schemes were grouped with the standard Zhou's prescription extract. These results demonstrated that this new method is an efficient and reliable approach for ensuring quality stability of Zhou's prescription extract.
    Analytical methods 01/2014; 6(12):4158. DOI:10.1039/c3ay41884g · 1.94 Impact Factor


Available from