Article

Development and identification of monoclonal antibodies against parathion.

Laboratory of Space Cellular & Molecular Biology, Institute of Space Medico-Engineering, Beijing, PR China.
Hybridoma (2005) (impact factor: 0.42). 01/2006; 24(6):298-304. DOI:10.1089/hyb.2005.24.298 pp.298-304
Source: PubMed

ABSTRACT An amino-derivative of parathion was prepared and conjugated to human serum albumin (HSA) and bovine thyroglobulin (BTG) via diazonium condensation. Spleen cells producing high titer antibody were removed and fused with myeloma cells of SP2/0 origin. Using a conventional immunization protocol, we generated nine stable murine monoclonal antibodies (MAbs) producing cell lines to parathion. After four successive limiting dilutions, antibodies produced by nine clones had high affinities, ranging from 10(9) to 10(12) M(-1). These clones were found to be of IgG class and IgM class with k light chain. Subclass determination showed that the clones produced IgG(1), IgG(2a), IgG(2b), and IgM types of antibody. One clone (2H(9)) was used to establish the calibration curve with a sensitivity of 26 ng/mL, a practical working range of 46.8-6000 ng/mL parathion.

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    Article: Specific antibody for pesticide residue determination produced by antibody-pesticide complex.
    Yi H Liu, Jian Chen, Chun M Wang, Yi R Guo, Xiao Liang, Guo N Zhu
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    ABSTRACT: A new method for specific antibody production was developed using antibody (Ab)-pesticide complex as a unique immunogen. Parathion (PA) was the targeted pesticide, and rabbit polyclonal antibody (Pab) and mouse monoclonal antibody (Mab) were used as carrier proteins. The Ab-PA complexes were generated by conjugating the two antibodies with an excessive dosage of PA. It was shown that the sensitivity of homologous enzyme-linked immunosorbent assay (ELISA) using the new antibodies was similar to that using original antibodies. However, the new mouse Pab had not only similar positive recognizing spectrum as the original Mab, but also a significantly improved sensitivity in heterologous ELISA when some recognizable competitors were applied. IC(50) value of ELISA based on a combination of the new mouse Pab and hapten 9 was 0.24 ng/mL, which was 445.54 times as that of the homologous ELISA. An Ab-pesticide complex may be a suitable alternative immunogen for producing highly specific antibody to improve the immunoassay sensitivity.
    Journal of Environmental Science and Health Part B Pesticides Food Contaminants and Agricultural Wastes 08/2010; 45(6):540-6. · 1.10 Impact Factor
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Keywords

amino-derivative
 
calibration curve
 
clone
 
clones
 
conventional immunization protocol
 
diazonium condensation
 
fused
 
HSA
 
human serum albumin
 
IgG class
 
IgM class
 
IgM types
 
k light chain
 
MAbs
 
stable murine monoclonal antibodies
 
Subclass determination
 
successive
 
titer antibody