Article

Leptin is an eosinophil survival factor

Department of Pharmacology, University of Bern, Switzerland.
Journal of Allergy and Clinical Immunology (Impact Factor: 11.25). 01/2006; 116(6):1228-34. DOI: 10.1016/j.jaci.2005.09.003
Source: PubMed

ABSTRACT Leptin regulates food intake, as well as metabolic, endocrine, and immune functions. It exerts proliferative and antiapoptotic activities in a variety of cell types, including T cells. Leptin also stimulates macrophages and neutrophils, and its production is increased during inflammation.
We sought to examine the expression of leptin receptors on eosinophils and the effect of recombinant leptin on proapoptotic pathways in these cells.
The presence of leptin receptor was examined by means of RT-PCR and immunofluorescence analysis in freshly isolated blood eosinophils and tissue eosinophils. The effect of recombinant leptin on apoptotic pathways in eosinophils was studied by using flow cytometric, immunoblotting, and immunofluorescence techniques.
Human eosinophils express leptin surface receptors under in vitro and in vivo conditions, and leptin delays apoptosis of mature eosinophils in vitro. The antiapoptotic effects of leptin were concentration dependent and blocked by an anti-leptin receptor mAb. The efficacy of leptin to block eosinophil apoptosis was similar to that of GM-CSF. Leptin delayed the cleavage of Bax, as well as the mitochondrial release of cytochrome c and second mitochondria-derived activator of caspase, suggesting that it blocks proapoptotic pathways proximal to mitochondria in eosinophils. Using pharmacological inhibitors, we obtained evidence that leptin initiates a signaling cascade involving phosphatidylinositol-3-OH kinase and mitogen-activated protein kinase-dependent pathways in eosinophils.
Leptin is a survival cytokine for human eosinophils, a finding with potential pathologic relevance in allergic and parasitic diseases.

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    • "For instance some members of the Bcl-2 family are involved in eosinophil survival. Specifically , increased expression of Bcl-X L (Dibbert et al., 1998), maintained Bid cleavage (Segal et al., 2007), inhibition of Bax translocation to the mitochondria (Dewson et al., 2001), and delayed Bax cleavage (Conus et al., 2005) result in maintained mitochondrial integrity and inhibition of caspase activation (Dewson et al., 2001; Conus et al., 2005). Likewise, neutrophils express anti-apoptotic members (Bcl-X L , Bcl2A1 and Mcl-1) as well as pro-apoptotic proteins (Bax, Bak, Bid, Bim and Puma) and those proteins regulate constitutive as well as inducible apoptosis/survival pathways (Weinmann et al., 1999; Moulding et al., 2001; Guo et al., 2006; Cowburn et al., 2011). "
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