Article

A detailed phenotypic and genotypic description of Pseudomonas strain OX1

Department of Biology, University of the Balearic Islands, Palma, Balearic Islands, Spain
Systematic and Applied Microbiology (Impact Factor: 3.31). 08/2006; 29(5):422-30. DOI: 10.1016/j.syapm.2005.11.007
Source: PubMed

ABSTRACT Strain OX1 exhibits important physiological, ecological, and biotechnological properties in the degradation of chemical pollutants. It was previously classified as a member of Pseudomonas stutzeri based on its phenotypic characteristics. The present taxonomic study describes phenotypic and genomic properties of strain OX1 and illustrates the value both of multigenic sequence analysis and siderotyping methods to justify its species circumscription within the genus Pseudomonas. We have concluded that strain OX1 is a member of the Pseudomonas corrugata group, distantly related to P. stutzeri, and should be considered representative of a new species. However, phenotypic differentiation between species in this group remains difficult, and species proposals based on only a single strain must be cautious. We, therefore, prefer not to propose a new species until more strains with the same genomic and phenotypic properties as strain OX1 have been isolated.

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    • "P. mediterranea CFBP5447 (T) gyrB: AM084678 (Ait Tayeb et al. 2005; Cladera et al. 2006) rpoD: AM084337 rpoB: AJ717449 P. corrugata CFBP2431 (T) gyrB: AB039460 (Yamamoto et al. 2000; Ait Tayeb et al. 2005) rpoD: AB039566 rpoB: AJ717487 The corresponding sequences for P. syringae pv. tomato, P. syringae pv. "
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    • "P. mediterranea CFBP5447 (T) gyrB: AM084678 (Ait Tayeb et al. 2005; Cladera et al. 2006) rpoD: AM084337 rpoB: AJ717449 P. corrugata CFBP2431 (T) gyrB: AB039460 (Yamamoto et al. 2000; Ait Tayeb et al. 2005) rpoD: AB039566 rpoB: AJ717487 The corresponding sequences for P. syringae pv. tomato, P. syringae pv. "
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    ABSTRACT: Recent taxonomic advances, based on biochemical and genotypic processes demonstrate that the plant pathogenic species Pseudomonas cichorii consists of a cluster of closely related genomic groups. Prior to this study, three morphotype groups had been described (C1-C3), all sharing various phenotypic and biochemical characters but partially differing in their DNA content. All entities of the complex could cause disease among a variety of hosts, including lettuce, celery, chrysanthemum and others. In this study, we present the biochemical and molecular characterization of P. cichorii isolates as the causal agent of pith necrosis of tomato plants. A detailed characterization of the genetic variability among strains belonging to P. cichorii was achieved using BOX-PCR and Multi Locus Sequence Analysis utilizing three housekeeping genes (gyrB, rpoD, rpoB). In addition, a number of biochemical and physiological tests were also used for the identification of the tomato P. cichorii isolates. To our knowledge, this is the first complete biochemical, molecular and phylogenetic study of P. cichorii strains isolated from tomato plants affected by pith necrosis disease. Our findings demonstrate the emergence of a new genomovar of P. cichorii, yet another indication for the genetic heterogeneity of the species.
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    • "P. mediterranea CFBP5447 (T) gyrB: AM084678 (Ait Tayeb et al. 2005; Cladera et al. 2006) rpoD: AM084337 rpoB: AJ717449 P. corrugata CFBP2431 (T) gyrB: AB039460 (Yamamoto et al. 2000; Ait Tayeb et al. 2005) rpoD: AB039566 rpoB: AJ717487 The corresponding sequences for P. syringae pv. tomato, P. syringae pv. "
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