Immune response induced by Salmonella typhimurium defective in ppGpp synthesis

Genome Research Center for Enteropathogenic Bacteria and Research Institute of Vibrio Infection, South Korea.
Vaccine (Impact Factor: 3.49). 04/2006; 24(12):2027-34. DOI: 10.1016/j.vaccine.2005.11.031
Source: PubMed

ABSTRACT Systemic infection by Salmonella typhimurium requires coordinated expression of virulence genes found primarily in Salmonella Pathogenecity Islands (SPIs). We have previously reported that the intracellular signal that induces these virulence genes is a stringent signal molecule, ppGpp [Song et al. J Biol Chem 2003;279:34183]. In this study, we found that relA and spoT double mutant Salmonella (DeltappGpp strain), which is defective in ppGpp synthesis, was virtually avirulent in BALB/c mice. Subsequently, the live vaccine potential of the avirulent DeltappGpp Salmonella strain was determined. A single immunization with live DeltappGpp Salmonella efficiently protected mice from challenge with wild-type Salmonella at a dose 10(6)-fold above the LD50 30 days after immunization. Various assays revealed that immunization of mice with the DeltappGpp strain elicited both systemic and mucosal antibody responses, in addition to cell-mediated immunity.

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    • "The attenuated S. typhimurium strains SHJ2037 and A1R were cultured in Luria-Bertani (LB) broth medium (Difco Laboratories, USA) with vigorous aeration at 37°C, as previously described (Na et al., 2006; Zhao et al., 2006). SHJ2037 (relA::cat, spoT::kan) is a ∆ppGpp strain and A1R is an auxotrophic mutant for leucine and arginine, kindly provided by R. M. Hoffman (AntiCancer, Inc., USA) (Zhao et al., 2006). "
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