Development of analytical methods for multiresidue determination of quinolones in pig muscle samples by liquid chromatography with ultraviolet detection, liquid chromatography-mass spectrometry and liquid chromatography-tandem mass spectrometry.
ABSTRACT This paper presents a comparison between liquid chromatography with ultraviolet detection (LC-UV), liquid chromatography-mass spectrometry (LC-MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods developed for the multiresidue determination of 8 quinolones, around their maximum residue levels (MRLs) in pig muscle. The procedure involves common extraction of the quinolones from the tissues by traditional extraction, a step for clean-up and preconcentration of the analytes by solid-phase extraction (SPE) and a subsequent liquid chromatographic analysis. The methods present satisfactory results of linearity, precision and limits of quantification much lower than the MRLs established by the European Union for quinolones in pig tissues.
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ABSTRACT: This work presents a comparison between three analytical methods developed for the simultaneous determination of eight quinolones regulated by the European Union (marbofloxacin, ciprofloxacin, danofloxacin, enrofloxacin, difloxacin, sarafloxacin, oxolinic acid and flumequine) in pig muscle, using liquid chromatography with fluorescence detection (LC–FD), liquid chromatography–mass spectrometry (LC–MS) and liquid chromatography-tandem mass spectrometry (LC–MS/MS). The procedures involve an extraction of the quinolones from the tissues, a step for clean–up and preconcentration of the analytes by solid-phase extraction and a subsequent liquid chromatographic analysis. The limits of detection of the methods ranged from 0.1 to 2.1 ng g−1 using LC–FD, from 0.3 to 1.8 using LC–MS and from 0.2 to 0.3 using LC–MS/MS, while inter- and intra-day variability was under 15 % in all cases. Most of those data are notably lower than the maximum residue limits established by the European Union for quinolones in pig tissues. The methods have been applied for the determination of quinolones in six different commercial pig muscle samples purchased in different supermarkets located in the city of Granada (south-east Spain).Chromatographia 76(11-12). · 1.44 Impact Factor
Article: B. SAN MARTÍN, J. CORNEJO, D. IRAGÜEN, H. HIDALGO and A. ANADÓN (2007). Depletion study of Enrofloxacin and its metabolite Ciprofloxacin in edible tissues and feathers of white leghorn hens by liquid chromatography coupled tandem mass spectrometry. Journal of Food Protection 70 (8), 1952-1957.01/2007;