Article

Spatio-temporal patterns of intestine-specific transcription factor expression during postnatal mouse gut development.

Division of Pediatric Gastroenterology, Stanford University School of Medicine, 750 Welch Road, Suite 116, Palo Alto, CA 94304, USA.
Gene Expression Patterns (impact factor: 2.02). 05/2006; 6(4):426-32. DOI:10.1016/j.modgep.2005.09.003 pp.426-32
Source: PubMed

ABSTRACT The small intestine matures from a primitive tube into morphologically and functionally distinct regions during gut development. Maximal expression of the genes encoding the digestive enzymes lactase-phlorizin hydrolase and sucrase-isomaltase is spatially restricted to distinct segments along the anterior-posterior axis of the small intestine and is temporally regulated during postnatal maturation. Transcription factors capable of interacting with the intestinal lactase and sucrase gene promoters are candidate regulators of spatio-temporal patterning during gut development and maturation. We aimed to quantitatively examine and compare the relative expression levels of a set of intestine-specific transcription factors along the anterior-posterior gut axis during postnatal maturation. Our analysis was focused on the transcription factors capable of regulating the intestinal lactase and sucrase-isomaltase genes. A real-time PCR protocol was used to quantitatively examine and compare spatially and temporally the relative transcript abundance levels for intestine-specific factors during postnatal intestinal maturation. Distinct spatial expressions patterns were detected along the length of the small intestine for PDX-1, Cdx-2, GATA-4, GATA-5, GATA-6, HNF-1alpha, HNF-1beta and CDP transcription factor genes. There is a general decline in transcript abundance for the factor genes during postnatal maturation. Defining the spatio-temporal expression patterns for intestine-specific transcription factor genes contributes to investigation of the roles that factor gradients play in mediating gut development and differentiation.

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Keywords

anterior-posterior gut axis
 
CDP transcription factor genes
 
digestive enzymes lactase-phlorizin hydrolase
 
Distinct spatial expressions patterns
 
functionally distinct regions
 
general decline
 
intestinal lactase
 
intestine-specific transcription factors
 
Maximal expression
 
mediating gut development
 
postnatal intestinal maturation
 
real-time PCR protocol
 
relative expression levels
 
relative transcript abundance levels
 
small intestine matures
 
spatio-temporal expression patterns
 
sucrase gene promoters
 
sucrase-isomaltase genes
 
transcript abundance
 
transcription factors capable