Dengue viral infection is one of the most important public health problem in tropical countries.
An outbreak of dengue fever was investigated in a periurban slum area of Chandigarh, India, during September to December, 2002.
Blood samples from 218 patients and 30 apparently healthy contacts were tested for dengue-specific immunoglobulin M (IgM) and IgG antibodies including 80 acute samples collected within 5 days of illness were subjected for virus isolation in newborn mice. The average temperature, rainfall, and humidity of the epidemic year were compared with the number of dengue cases.
statistical significance was found out using c2-test.
A total of 76 cases were positive by either dengue IgM capture assay (n = 57) or virus isolation (n = 17) or both (n = 2). Fifteen of nineteen viral isolates subjected for typing by type-specific multiplex reverse transcription-polymerase chain reaction were found to be of dengue virus. High rainfall and humidity with the temperature range from 21 degrees C to 33 degrees C during the months of August and September might have favored the breeding of mosquitoes, thus leading to an increase in the number of dengue cases in October and November, 2002.
The present outbreak thus emphasizes the need for continuous sero epidemiological and entomological surveillance for the timely implementation of effective dengue control programme.
"Several studies have linked poverty or wealth to dengue (Lifson, 1996; Ratho et al., 2005; Flauzino et al., 2009; Mena et al., 2011), while others have found only transitory associations (Heukelbach et al., 2001; Mondini and Chiaravalloti Neto, 2007), highlighting the complexity of this relationship. It is possible that using relative poverty instead of absolute poverty might better discriminate between levels of economic disadvantage and detect disparities not only between urban and rural environments, but also within them. "
"In our study 35.5% of cases were serologically positive for dengue infection. The higher positivity among young adult males (61%) is consistent with previous dengue reports by the authors, as well as other Indian studies. In several other studies pediatric population was most commonly affected. "
[Show abstract][Hide abstract] ABSTRACT: Introduction:Dengue is one of the most important arboviral infections caused by one of the four dengue serotypes, 1-4.Objective:To study the applicability of different diagnostic methods in diagnosis of dengue viral infection.Materials and Methods:A total of 2101 blood samples were collected for confirmation of dengue viral infection. All the samples were tested by dengue-specific IgM ELISA, of which 111 were also tested for NS1 antigen detection and 27 acute samples (≤5 days) were further subjected for viral RNA detection by RT-PCR and isolation in C6/36 cell line. To detect the sensitivity of NS1 antigen for different dengue virus serotypes, four dengue serotype 1 and 12 dengue 3 were subjected for the NS1 antigen assay.Results:Most common age group affected was 16-45 years, with male to female ratio of 2.8:1. During first 3 days of illness virus isolation and RT-PCR were the most sensitive (83%) followed by NS1 antigen detection (75%) and IgM detection (37.5%). The positivity of IgM detection was found to be significantly higher as compared to NS1 detection during 4 to 5 days and also after 5 days of illness (P < 0.05). Dengue serotypes 1 and 3 were found to be co-circulated, dengue 1 being the predominant serotype.Conclusion:Virus isolation and RT-PCR were the most sensitive tests during the early period of illness whereas beyond third day, IgM antibody detection was found to be the most sensitive method of dengue diagnosis.
Journal of global infectious diseases 07/2014; 6(3):109-13. DOI:10.4103/0974-777X.138504
"The extracted viral RNA was reverse transcribed to cDNA using murine Moloney leukemia virus reverse transcriptase (MBI Fermentas, USA) following the manufacturer's instructions and stored at −20 °C. The cDNA of all the cases and controls was subjected to dengue nested RT-PCR and chikungunya RT-PCR using DENV-and CHIKV-specific primers, respectively (Lanciotti et al., 1992; Naresh et al., 2007; Ratho et al., 2005). "
[Show abstract][Hide abstract] ABSTRACT: The reemergence of chikungunya virus (CHIKV) has compounded the already existing dengue problem because of clinical similarities and common vector, demanding the need for a rapid and specific diagnosis. Thus, dengue chikungunya multiplex reverse transcriptase-polymerase chain reaction (DCmRT-PCR) was developed and validated for simultaneous detection of dengue and chikungunya viral infections and its utility in virus serotyping. Blood samples from 97 suspected dengue and chikungunya cases and 10 healthy controls were subjected to dengue and chikungunya conventional RT-PCR and DCmRT-PCR. Thirty-one of 97 samples were positive for dengue or chikungunya viral RNA by RT-PCR and DCmRT-PCR with 100% concordance. DCmRT-PCR products were cycle sequenced. Seven dengue virus strains were clustered within genotype III of DENV-3 and 4 within genotype III of DENV-1, whereas chikungunya sequences were clustered within the Central/East African genotype. DCmRT-PCR was found to be a potential rapid test for simultaneous detection of dengue and CHIKV in clinical samples along with dengue serotyping.
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