An outbreak of dengue fever in Peri Urban slums of Chandigarh, India, with special reference to entomological and climatic factors

Department of Virology, Post Graduate Institute of Medical Education & Research, General Hospital, Sector-16, Chandigarh., India.
Indian Journal of Medical Sciences (Impact Factor: 1.67). 01/2006; 59(12):518-26. DOI: 10.4103/0019-5359.19194
Source: PubMed


Dengue viral infection is one of the most important public health problem in tropical countries.
An outbreak of dengue fever was investigated in a periurban slum area of Chandigarh, India, during September to December, 2002.
Blood samples from 218 patients and 30 apparently healthy contacts were tested for dengue-specific immunoglobulin M (IgM) and IgG antibodies including 80 acute samples collected within 5 days of illness were subjected for virus isolation in newborn mice. The average temperature, rainfall, and humidity of the epidemic year were compared with the number of dengue cases.
statistical significance was found out using c2-test.
A total of 76 cases were positive by either dengue IgM capture assay (n = 57) or virus isolation (n = 17) or both (n = 2). Fifteen of nineteen viral isolates subjected for typing by type-specific multiplex reverse transcription-polymerase chain reaction were found to be of dengue virus. High rainfall and humidity with the temperature range from 21 degrees C to 33 degrees C during the months of August and September might have favored the breeding of mosquitoes, thus leading to an increase in the number of dengue cases in October and November, 2002.
The present outbreak thus emphasizes the need for continuous sero epidemiological and entomological surveillance for the timely implementation of effective dengue control programme.

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    • "Several studies have linked poverty or wealth to dengue (Lifson, 1996; Ratho et al., 2005; Flauzino et al., 2009; Mena et al., 2011), while others have found only transitory associations (Heukelbach et al., 2001; Mondini and Chiaravalloti Neto, 2007), highlighting the complexity of this relationship. It is possible that using relative poverty instead of absolute poverty might better discriminate between levels of economic disadvantage and detect disparities not only between urban and rural environments, but also within them. "

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    • "The extracted viral RNA was reverse transcribed to cDNA using murine Moloney leukemia virus reverse transcriptase (MBI Fermentas, USA) following the manufacturer's instructions and stored at −20 °C. The cDNA of all the cases and controls was subjected to dengue nested RT-PCR and chikungunya RT-PCR using DENV-and CHIKV-specific primers, respectively (Lanciotti et al., 1992; Naresh et al., 2007; Ratho et al., 2005). "
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