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A selective turn-on fluorescent sensor for imaging copper in living cells.

Li Zeng, Evan W Miller, Arnd Pralle, Ehud Y Isacoff, Christopher J Chang

Department of Chemistry, University of California, Berkeley, CA 94720, USA.

Journal Article: Journal of the American Chemical Society (impact factor: 8.58). 02/2006; 128(1):10-1. DOI: 10.1021/ja055064u

Abstract

We present the synthesis, properties, and biological applications of Coppersensor-1 (CS1), a new water-soluble, turn-on fluorescent sensor for intracellular imaging of copper in living biological samples. CS1 utilizes a BODIPY reporter and thioether-rich receptor to provide high selectivity and sensitivity for Cu+ over other biologically relevant metal ions, including Cu2+, in aqueous solution. This BODIPY-based probe is the first Cu+-responsive sensor with visible excitation and emission profiles and gives a 10-fold turn-on response for detecting this ion. Confocal microscopy experiments further establish that CS1 is membrane-permeable and can successfully monitor intracellular Cu+ levels within living cells.

Source: PubMed

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Keywords

10-fold turn-on response
 
aqueous solution
 
biologically relevant metal ions
 
BODIPY-based probe
 
Confocal microscopy experiments
 
Coppersensor-1
 
detecting
 
emission profiles
 
first Cu+-responsive sensor
 
new water-soluble
 
selectivity
 
turn-on fluorescent sensor
 
visible excitation