Article

A study of chronic dermatophyte infection in a rural hospital.

Indian Journal of Dermatology Venereology and Leprology (Impact Factor: 1.21). 71(2):129-30.
Source: PubMed
0 0
 · 
0 Bookmarks
 · 
52 Views
  • Source
    [show abstract] [hide abstract]
    ABSTRACT: Piper longum L. (Piperaceae) commonly known as ‘‘long pepper’’ is awell known medicinal plant in ayurveda. Different parts of this plant, such as root, seed, fruit, whole plant etc. are used traditionally in various ailments. Here we have investigated the antidermatophytic activity of sequentially extracted petroleum ether, chloroform, methanol and water extracts from P. longum leaf against Trichophyton mentagrophytes, T. rubrum, T. tonsurans, Microsporum fulvum and M. gypseum. Better activity of chloroform and methanol extracts was observed. The chloroform extractwas selected for further study and theMICvaluewas recorded as 5.0 mg ml-1 against the test organisms. In the chloroform extract, tannins and phenolic compounds were detected. Further activity-guided fractionation of chloroform extract by silica gel column chromatography yielded nine major fractions. Among these, fraction-1, 4, 5 and 7 showed higher antidermatophytic activity. Fraction-4 on further purification by repeated column chromatography yielded a potential antidermatophytic fraction showing MIC value of 0.625 mg ml-1 against T. mentagrophytes and T. rubrum as determined by broth microdilution method. The major compounds were identified as 1,2-benzenedicarboxylic acid, bis(2-ethylhexyl) ester (C24H38O4] (41.45 %), 2,2-dimethoxybutane (C6H14O2] (13.6 %) and b-myrcene (C10H16) (6.75 %) based on GC–MS data.
    Indian Journal of Microbiology. 01/2012;
  • Source
    [show abstract] [hide abstract]
    ABSTRACT: Piper longum L. (Piperaceae) commonly known as ‘‘long pepper’’ is awell known medicinal plant in ayurveda.Different parts of this plant, such as root, seed, fruit, whole plant etc. are used traditionally in various ailments. Here we have investigated the antidermatophytic activity of sequentially extracted petroleum ether, chloroform, methanol and water extracts from P. longum leaf against Trichophyton mentagrophytes, T. rubrum, T. tonsurans, Microsporum fulvum and M. gypseum. Better activity of chloroform and methanol extracts was observed. The chloroform extractwas selected for further study and theMICvaluewas recorded as 5.0 mg ml-1 against the test organisms. In the chloroform extract, tannins and phenolic compounds were detected. Further activity-guided fractionation of chloroform extract by silica gel column chromatography yielded nine major fractions. Among these, fraction-1, 4, 5 and 7 showed higher antidermatophytic activity. Fraction-4 on further purification by repeated column chromatography yielded a potential antidermatophytic fraction showing MIC value of 0.625 mg ml-1 against T. mentagrophytes and T. rubrum as determined by broth microdilution method. The major compounds were identified as 1,2-benzenedicarboxylic acid,bis(2-ethylhexyl) ester (C24H38O4] (41.45 %), 2,2-dimethoxybutane (C6H14O2] (13.6 %) and b-myrcene (C10H16) (6.75 %) based on GC–MS data
    Indian Journal of Microbiology 08/2012; 52(4):624-629. · 0.46 Impact Factor

P V S Prasad