A study of chronic dermatophyte infection in a rural hospital.
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ABSTRACT: Piper longum L. (Piperaceae) commonly known as ‘‘long pepper’’ is awell known medicinal plant in ayurveda.Different parts of this plant, such as root, seed, fruit, whole plant etc. are used traditionally in various ailments. Here we have investigated the antidermatophytic activity of sequentially extracted petroleum ether, chloroform, methanol and water extracts from P. longum leaf against Trichophyton mentagrophytes, T. rubrum, T. tonsurans, Microsporum fulvum and M. gypseum. Better activity of chloroform and methanol extracts was observed. The chloroform extractwas selected for further study and theMICvaluewas recorded as 5.0 mg ml-1 against the test organisms. In the chloroform extract, tannins and phenolic compounds were detected. Further activity-guided fractionation of chloroform extract by silica gel column chromatography yielded nine major fractions. Among these, fraction-1, 4, 5 and 7 showed higher antidermatophytic activity. Fraction-4 on further purification by repeated column chromatography yielded a potential antidermatophytic fraction showing MIC value of 0.625 mg ml-1 against T. mentagrophytes and T. rubrum as determined by broth microdilution method. The major compounds were identified as 1,2-benzenedicarboxylic acid,bis(2-ethylhexyl) ester (C24H38O4] (41.45 %), 2,2-dimethoxybutane (C6H14O2] (13.6 %) and b-myrcene (C10H16) (6.75 %) based on GC–MS dataIndian Journal of Microbiology 08/2012; 52(4):624-629. DOI:10.1007/s12088-012-0303-x · 0.83 Impact Factor
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ABSTRACT: The present study was executed to screen methanolic leaf extracts from 19 plant species for antidermatophytic activity. Based on highest activity, Piper longum was further fractionated to identify the fractions of better activity profile. The extracts and fractions were tested against five dermatophyte species by agar well diffusion and agar dilution methods. Extracts from Aloe vera, Azadirachta indica, Camelia sinensis, Cassia sophera, Clitoria ternatea, Leucas plukentii, Lawsonia inermis, Ocimum basillicum, O. sanctum, O. gratissimum, P. betleoides, P. longum, Solanum melongena and Vitex negundo showed antidermatophytic activity (zone of inhibition: 9-42 mm, MIC: 1.25 -10 × 10 4 μ μ μ μ μg mL -1). Furthermore bioassay of P. longum exhibited better activity of sequentially extracted chloroform and methanol extracts (MIC: 5 × 10 3 μ μ μ μ μg mL -1). Two potential column fractions isolated from the methanol extract of P. longum showed MIC of 1.25 × 10 3 -2.5 × 10 3 µg mL -1 in broth microdilution assay. To the best of our knowledge, there is no scientific report on antidermatophytic activity of P. longum so far. The results highlighted future research on these potential plants especially P. longum to characterize the active constituents.