[Show abstract][Hide abstract] ABSTRACT: Piper longum L. (Piperaceae) commonly known as ‘‘long
pepper’’ is awell known medicinal plant in ayurveda.Different
parts of this plant, such as root, seed, fruit, whole plant etc. are
used traditionally in various ailments. Here we have investigated
the antidermatophytic activity of sequentially extracted
petroleum ether, chloroform, methanol and water extracts
from P. longum leaf against Trichophyton mentagrophytes,
T. rubrum, T. tonsurans, Microsporum fulvum and M. gypseum.
Better activity of chloroform and methanol extracts was
observed. The chloroform extractwas selected for further study
and theMICvaluewas recorded as 5.0 mg ml-1 against the test
organisms. In the chloroform extract, tannins and phenolic
compounds were detected. Further activity-guided fractionation
of chloroform extract by silica gel column chromatography
yielded nine major fractions. Among these, fraction-1, 4, 5
and 7 showed higher antidermatophytic activity. Fraction-4 on
further purification by repeated column chromatography yielded
a potential antidermatophytic fraction showing MIC value
of 0.625 mg ml-1 against T. mentagrophytes and T. rubrum
as determined by broth microdilution method. The major
compounds were identified as 1,2-benzenedicarboxylic acid,bis(2-ethylhexyl) ester (C24H38O4] (41.45 %), 2,2-dimethoxybutane
(C6H14O2] (13.6 %) and b-myrcene (C10H16)
(6.75 %) based on GC–MS data
Indian Journal of Microbiology 08/2012; 52(4):624-629. DOI:10.1007/s12088-012-0303-x · 0.90 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: The present study was executed to screen methanolic leaf extracts from 19 plant species for antidermatophytic activity. Based on highest activity, Piper longum was further fractionated to identify the fractions of better activity profile. The extracts and fractions were tested against five dermatophyte species by agar well diffusion and agar dilution methods. Extracts from Aloe vera, Azadirachta indica, Camelia sinensis, Cassia sophera, Clitoria ternatea, Leucas plukentii, Lawsonia inermis, Ocimum basillicum, O. sanctum, O. gratissimum, P. betleoides, P. longum, Solanum melongena and Vitex negundo showed antidermatophytic activity (zone of inhibition: 9-42 mm, MIC: 1.25 -10 × 10 4 μ μ μ μ μg mL -1). Furthermore bioassay of P. longum exhibited better activity of sequentially extracted chloroform and methanol extracts (MIC: 5 × 10 3 μ μ μ μ μg mL -1). Two potential column fractions isolated from the methanol extract of P. longum showed MIC of 1.25 × 10 3 -2.5 × 10 3 µg mL -1 in broth microdilution assay. To the best of our knowledge, there is no scientific report on antidermatophytic activity of P. longum so far. The results highlighted future research on these potential plants especially P. longum to characterize the active constituents.
Journal of Pure and Applied Microbiology 12/2013; 7(4):3207-3212. · 0.07 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Introduction: Dermatophytic infections are commonly encountered a problem and constitute more than 50% of cases in dermatology outpatient departments. Diagnosis of these infections requires the proper use of laboratory methods. Objectives: This study was conducted to know the etiology of dermatophytosis in patients attending Tertiary Care Level Hospital in South India and to compare the efficacy of Sabouraud’s dextrose agar (SDA) with actidione and dermatophyte test medium (DTM) in isolating and identifying dermatophytes. Materials and Methods: A total of 110 samples which included 101 skin samples and 9 hair samples from clinically suspected dermatophytosis were collected. Direct microscopy by KOH and culture on SDA with actidione and DTM were done. Results: Of 110 samples collected, 58.18% were KOH positive for fungal filaments and 56.36% were culture positive for dermatophytes. More number of cases were observed between age groups of 21–40 years. Males were more affected compared to females. Tinea corporis was the common clinical presentation observed (40%). Trichophyton rubrum (58.06%) was the predominant isolate recovered in all clinical presentations but Trichophyton violaceum was the most common isolate in tinea capitis. All culture positives were grown on both SDA with actidione and DTM. Appearance of growth was earlier on DTM that is, within 10 days compared to SDA with actidione where growth started appearing only after 10 days. This is statistically significant P < 0.0001 (c2 = 71.6). Species level identification on primary isolation was possible when grown on SDA with actidione and it was not possible with the growth on DTM on primary isolation. Conclusion: DTM is a good screening medium in laboratory diagnosis of dermatophytosis when compared to SDA with actidione. But DTM is inferior to SDA with actidione in identification of dermatophyte species.
Key words: Dermatophte test medium, dermatophytosis, Sabouraud’s dextrose agar with actidione
Journal of laboratory physicians 07/2015; volume 7(issue 2):page 84-89. DOI:10.4103/0974-2727.163135
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