Article

Immunohistochemical Staining for Claudin-1 Can Help Distinguish Meningiomas From Histologic Mimics

Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA.
American Journal of Clinical Pathology (Impact Factor: 3.01). 03/2006; 125(2):203-8. DOI: 10.1309/G659-FVVB-MG7U-4RPQ
Source: PubMed

ABSTRACT The histologic distinction between meningiomas and other tumors of the central nervous system occasionally can be difficult. Claudin-1 is a tight junction-associated protein recently shown to be expressed in anaplastic meningiomas. The purpose of this study was to determine whether immunohistochemical staining for claudin-1 could help distinguish meningiomas from histologic mimics, compared with commonly used markers. Tissue sections from 10 meningothelial meningiomas, 20 fibrous meningiomas, 10 atypical meningiomas, 7 solitary fibrous tumors of the meninges, 5 meningeal hemangiopericytomas, and 7 vestibular schwannomas were stained immunohistochemically for claudin-1, epithelial membrane antigen, S-100 protein, CD34, and glial fibrillary acidic protein. In total, 21 (53%) of 40 meningiomas were immunoreactive for claudin-1, whereas none of the other tumors were positive. In contrast, there was considerable overlap in the distribution of the other antibodies evaluated. Claudin-1 seems to be a specific marker for meningiomas in this context. Although its sensitivity is relatively low, claudin-1 may be helpful in a panel of immunostains to distinguish meningiomas from histologic mimics.

0 Followers
 · 
88 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: Diagnostic neuro-oncology practice has benefited in the last 2 decades from the incorporation of, and recent advances in, immunohistochemistry (IHC). Although the mainstay of brain tumor diagnosis including its grading remains conventional hematoxylin-eosin (H&E)-stained histology, it is no doubt that IHC plays a major role in differential diagnosis and in improving the diagnostic accuracy. It is generally used for purposes of (1) identifying tumor cell type/origin, (2) assessing cell proliferation potential, (3) evaluating the boundary between tumor and the surrounding tissue (i.e., tumor “margin”), and (4) excluding reactive processes (e.g., demyelinating and infectious diseases). In the future, novel antibodies utilized as prognostic, predictive markers and as potential therapeutic targets for brain tumors will be developed.
  • [Show abstract] [Hide abstract]
    ABSTRACT: An understanding of the cutting process and its mathematical modelling requires knowledge about the material structures and their behaviours under the mechanical and thermal loads in the transformation zone. The tests with single and double phase microstructures - copper and carbon steels, with preliminary deformed and undeformed structures have been executed. The influence of various microstructures and process conditions on the energy quanta and the plastic deformation temperature in the transformation zone and the dislocation mechanism are better understood. Preliminary accumulated energy in the material structure contributed to the transformation energy and to a decrease of the temperature. Based of these results some elements of the transfer function of the process may contribute to a generic expression for an on line cutting process identification.
    CIRP Annals - Manufacturing Technology 01/1998; 47(1):83-86. DOI:10.1016/S0007-8506(07)62790-5 · 2.54 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: We analyzed the diagnostic role of claudins in effusion cytology in 325 effusions, including 218 ovarian, 49 breast, 15 cervical or endometrial, 10 gastrointestinal, and 8 lung adenocarcinomas and 25 malignant mesotheliomas (MMs). Specimens were analyzed for claudin-1 and claudin-3 expression using immunohistochemical analysis. Ovarian and breast adenocarcinoma were further analyzed for claudin-7 expression. Claudin-1 expression was most frequent in ovarian and cervical or endometrial adenocarcinoma compared with other adenocarcinomas and MMs (P < .001). Claudin-3 expression was comparable in adenocarcinomas of different origin but was absent in MMs (P < .001). Reactive mesothelial cells rarely expressed claudins. Claudin-7 expression was higher in ovarian than in breast adenocarcinoma (P < .001). Our data suggest that expression of claudin-3 or claudin-7 is specific for adenocarcinoma and rules out the diagnosis of cells as mesothelial and that absence of claudin-1 expression excludes ovarian carcinoma as the possible origin of metastatic adenocarcinoma. Claudins may, therefore, be of diagnostic value in effusion cytology.
    American Journal of Clinical Pathology 07/2007; 127(6):928-37. DOI:10.1309/V025QRN3R9CJGNPX · 3.01 Impact Factor
Show more

Preview

Download
0 Downloads
Available from