Ganoderma lucidum causes apoptosis in leukemia, lymphoma and multiple myeloma cells.
ABSTRACT Over many centuries, herbal remedies have treated a variety of ailments. This empiric observational approach has produced a number of leads for formulated medicines. Ganoderma lucidum extract was screened for its anti-proliferative activity using a panel of 26 human cancer cell lines. The six most sensitive hematologic cell lines were: HL-60 (ED50 26 microg/ml), U937 (63 microg/ml), K562 (50 microg/ml), Blin-1 (38 microg/ml), Nalm-6 (30 microg/ml) and RPMI8226 (40 microg/ml). Cell cycle analyses revealed a G2/M arrest, most prominently in HL-60 cells. Four hematopoietic cell lines (HL-60, Blin-1, U937, RPMI8226) were examined for apoptosis, which ranged between 21 and 92%. After exposure to G. lucidum extract, HL-60 cells became multinucleated with an increased DNA content. These results indicate that G. lucidum extract has a profound activity against leukemia, lymphoma and multiple myeloma cells and may be a novel adjunctive therapy for the treatment of hematologic malignancies.
SourceAvailable from: Sheng Zhuo Huang[Show abstract] [Hide abstract]
ABSTRACT: Chemical investigation of the fruiting bodies of Ganoderma tropicum led to the isolation of two new phenolic compounds, ganodermatropins A (1) and B (2). Their structures were elucidated by spectroscopic techniques (MS, 1D and 2D NMR). Ganodermatropin A exhibited antimicrobial activity against Staphylococcus aureus.Bulletin- Korean Chemical Society 03/2013; 34(3). DOI:10.5012/bkcs.2013.34.3.884 · 0.84 Impact Factor
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ABSTRACT: An ethanolic extraction of Ganoderma lucidum, a medicinal mushroom, was fractioned into four extracts by flash chromatography. Only fraction 2 and 3 contained triterpenoids. Fraction 2 was less polar and contained ganoderic acids ζ, A, B, C2, C6, D, G, H, I, K and ganoderenic acids D and K, all with presence of oxyl or hydroxyl groups at position C23. Fraction 3 contained ganoderic acid DM, ganoderic aldehyde TR and an unknown compound. None of identified compounds has functional groups at C23. Both fractions 2 and 3 inhibited Caco-2 cells in a dose dependent manner. The LC50 of fraction 2 and 3 were 0.528 ± 0.078 and 0.348 ± 0.032 mg/mL. A significant accumulation of sub-G1 cells (18.77 ± 4.37%) were found with fraction 3 but not with fraction 2. TUNEL assay resulted in 29.97 ± 3.03% apoptotic cells and caspase apoptotic assay showed 11.73 ± 2.05% mid-apoptotic cells and 16.03 ± 2.45% late apoptotic cells. The caspase apoptotic assay indicated an involvement of caspase enzyme family mediation. Two fractions were cytotoxic. Fraction 2 caused a G2/M arrest while fraction 3 induced apoptosis. These differences are likely a result of the polarity of the triterpenoids.07/2012; 2(3):203-209. DOI:10.1016/j.bionut.2012.03.004
Dataset: 2012 Leukemia NBE