Serological survey of vector-borne zoonotic pathogens in pet cats and cats from animal shelters and feral colonies
ABSTRACT Although cats and their arthropod parasites can sometimes be important sources of zoonotic diseases in humans, the extent of exposure among various cat populations to many potential zoonotic agents remains incompletely described. In this study, 170 domestic cats living in private homes, feral cat colonies, and animal shelters from California and Wisconsin were evaluated by serology to determine the levels of exposure to a group of zoonotic vector-borne pathogens. Serological positive test results were observed in 17.2% of cats for Rickettsia rickettsii, 14.9% for R akari, 4.9% for R typhi, 11.1% for R felis, and 14.7% for Bartonella henselae. Although vector-borne disease exposure has been documented previously in cats, the evaluation of multiple pathogens and diverse cat populations simultaneously performed here contributes to our understanding of feline exposure to these zoonotic pathogens.
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ABSTRACT: Rickettsiosis was first described in Colombia in 1937 by Dr Luis Patiño during an outbreak of a disease with unspecific signs. Rickettsia is a genus of Gram-negative intracellular obligatory bacteria having caused several epidemics around the world, and are transmitted mainly by ticks, fleas, lice and mites. The most fatal within this group of diseases is known as Rocky Mountain Spotted Fever (RMSF), which is caused by Rickettsia rickettsia. There is also the endemic typhus caused by Rickettsia typhi and epidemic typhus caused by Rickettsia prowazekii. In Colombia, several outbreaks of RMSF have occurred during the last decade. The best known among those have hit the municipalities of Necoclí and Turbo, in Antioquia in 2006 and 2008 respectively, and Los Cordobas in the department of Cordoba in 2007. The goal of this review is to describe the state of the art of rickettsiosis, a forgotten lethal disease that has re-emerged in our country, and leave some questions as an inspiration for future research that will hopefully lead scientists to a better understanding of this entity potentially endemic in some areas of Colombia.Universitas Scientiarum 04/2012; 17(1):82-99.
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ABSTRACT: Mediterranean Spotted Fever (MSF), whose etiological agent is R. conorii, is one of the oldest described vector-borne infectious diseases. Although it is endemic in the Mediterranean area, clinical cases have also been reported in other regions. R. massiliae-Bar29 is related to MSF cases. This strain is distributed worldwide. R. conorii and R. massiliae-Bar29 are transmitted by ticks. Dogs are considered the sentinel of R. conorii infection. Cats could also be involved in their transmission. Rickettsia felis, etiological agent of Flea-borne spotted fever, is mainly transmitted by the cat flea, Ctenocephalides felis. Up to now, the role of cats in its transmission is not entirely elucidated. The aim of the study is to analyze the infection in cats by these microorganisms.Parasites & Vectors 01/2014; DOI:10.1186/1756-3305-7-353 · 3.25 Impact Factor
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ABSTRACT: The present study evaluated the rickettsial infection in a laboratory colony of cat fleas, Ctenocephalides felis felis (Bouche) in Brazil. All flea samples (30 eggs, 30 larvae, 30 cocoons, 30 males, and 30 females) tested by polymerase chain reaction (PCR) were shown to contain rickettsial DNA. PCR products, corresponding to the rickettsial gltA, htrA, ompA and ompB gene partial sequences were sequenced and showed to correspond to Rickettsia felis, indicating that the flea colony was 100% infected by R. felis. The immunofluorescence assay (IFA) showed the presence of R. felis-reactive antibodies in blood sera of 7 (87.5%) out of 8 cats that were regularly used to feed the flea colony. From 15 humans that used to work with the flea colony in the laboratory, 6 (40.0%) reacted positively to R. felis by IFA. Reactive feline and human sera showed low endpoint titers against R. felis, varying from 64 to 256. With the exception of one human serum, all R. felis-reactive sera were also reactive to Rickettsia rickettsii and/or Rickettsia parkeri antigens at similar titers to R. felis. The single human serum that was reactive solely to R. felis had an endpoint titer of 256, indicating that this person was infected by R. felis.Brazilian Journal of Microbiology 07/2010; 41(3):813-8. DOI:10.1590/S1517-83822010000300035 · 0.45 Impact Factor