Monocyte chemotactic protein-1 in the follicle of the menstrual and IVF cycle
ABSTRACT Ovulation constitutes an inflammatory-like process, with macrophages migrating into the follicle. This study evaluates the production of two macrophage-specific chemokines, monocyte chemotactic protein-1 (MCP-1) and macrophage inflammatory protein-1alpha (MIP-1alpha), in the human follicle at ovulation. Blood samples, follicular fluids and follicular cells were collected during menstrual and IVF cycles. Levels of MCP-1 and MIP-1alpha were measured in follicular fluid, blood plasma and cultured media (granulosa, theca and granulosa-lutein cells [GLCs]). Cells were cultured with or without LH, FSH, interleukin (IL)-1alpha, IL-1beta, tumour necrosis factor (TNF) alpha, progesterone or estradiol. The levels of MCP-1 were markedly higher in follicular fluid as compared with blood plasma in both menstrual and IVF cycles. The difference in MCP-1 levels between follicular fluid and plasma in menstrual cycles increased from the follicular phase (three-fold difference) to the late ovulatory phase (25-fold). Levels of MIP-1alpha were low in plasma and follicular fluid of both menstrual and IVF cycles. Theca cells from follicles of menstrual cycles secreted both MCP-1 and MIP-1alpha under basal conditions, and the secretion was increased by addition of IL-1beta (MCP-1 and MIP-1alpha) and IL-1alpha (MCP-1). GLCs secreted MCP-1 under basal conditions and also MIP-1alpha after IL-1beta stimulation. The macrophage-specific chemokine MCP-1 is highly expressed and is induced by IL-1 in the theca layer of the human follicle at ovulation.
- SourceAvailable from: Péter Fedorcsák
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- "Third, GL cells secrete steroid hormones , which have pleiotropic effects on immune cells (Bouman et al., 2005). Four, ovarian granulosa cells secrete, beyond IL-8, additional chemokines that may periodically direct specific leukocyte subsets to the ovary, including monocyte chemoattractant protein-1, growth-regulated-a and fractalkine (Karströ m-Encrantz et al., 1998; Dahm-Kahler et al., 2006; Zhao et al., 2008). The ovary, besides placenta, is a unique site of physiological angiogenesis in adults. "
ABSTRACT: Leukocyte infiltration and angiogenesis in the forming corpus luteum are prerequisites for normal ovarian function and may also underlie disorders like ovarian hyperstimulation syndrome. We examined whether ovarian angiogenesis could be affected by an interaction between granulosa-lutein (GL) cells and leukocytes. We found that GL cells isolated from the follicular fluid synthesize and secrete the chemokine interleukin-8 (IL-8), which activates IL-8-receptor-specific Ca(2+) and p38 mitogen-activated protein kinase signalling in monocytes and induces a directed migration of these cells towards the chemical gradient. Monocytes were found to further enhance IL-8 release, which suggests that these cells promote a massive leukocyte infiltration of the forming corpus luteum. A possible utility of leukocyte infiltration is the modulation of angiogenesis. We found that GL cells induce migration and capillary tube formation by endothelial cells in vitro. Furthermore, monocytes altered the profile of angiogenic factors released by GL cells, which supports the theory that an interaction between GL cells and leukocytes regulates ovarian angiogenesis. In addition, we found a correlation between increased secretion of pro-angiogenic cytokines and number of oocytes collected during IVF, which suggests that ovarian angiogenesis is related to the clinical response during ovarian stimulation. An intricate communication may exist between infiltrating leukocytes and ovarian GL cells during the formation of corpus luteum, affecting neo-vascularization of the luteal tissue.Human Reproduction 08/2011; 26(10):2819-29. DOI:10.1093/humrep/der216 · 4.59 Impact Factor
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ABSTRACT: A method for introducing inhibitory weights into RAM based nets has been developed. The inhibitory weights leads to a more robust net and much lower error rates can be obtained. In the paper we describe how the inhibition factors can be learned with a one shot learning scheme. The main strategy is to choose the inhibitory values so that they minimise the error-rate obtained in a crossvalidating test performed on the training set. The inhibition technique has been tested on the task of recognising handwritten digits. The results obtained match the best error rates reported in the literatureNeural Networks for Identification, Control, Robotics, and Signal/Image Processing, 1996. Proceedings., International Workshop on; 09/1996