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Apple Procyanidin Oligomers Absorption in Rats after Oral Administration: Analysis of Procyanidins in Plasma Using the Porter Method and High-Performance Liquid Chromatography/Tandem Mass Spectrometry

National Institute of Health Sciences, Japan, Edo, Tōkyō, Japan
Journal of Agricultural and Food Chemistry (Impact Factor: 3.11). 03/2006; 54(3):884-92. DOI: 10.1021/jf052260b
Source: PubMed

ABSTRACT In this study, we investigated the absorption of apple procyanidins, namely, apple condensed tannins (ACTs), in rats using the Porter method and high-performance liquid chromatography/tandem mass spectrometry. The apple procyanidin concentrations in the rat plasma reached a maximum 2 h after administration and decreased thereafter. To investigate the limits of the absorption of apple procyanidins in the polymerization degree, we administered the procyanidin oligomer fraction, which was separated from ACT using normal-phase chromatography according to the degree of polymerization. Procyanidins from each dimer to pentamer group were detected in the plasma by the Porter method. Moreover, by the study using reconstituted procyanidins, polymeric procyanidins influenced the absorption of procyanidin oligomers. These results suggest that ACTs are absorbed and directly involved in physiological functions in the rats.

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    • "Lower-molecularweight components and metabolites are more easily absorbed (Fernandes et al., 2012; Kosi nska and Andlauer, 2012). Most in vivo studies suggest that procyanidin dimers can be absorbed (Baba et al., 2002; Tsang et al., 2005; Shoji et al., 2006; Prasain et al., 2009). The gut is the predominant location for absorption, a large percentage of the parent compound may be transformed by gut microflora, and the resulting metabolites may constitute the predominant form of absorption (Stoupi et al., 2010). "
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    • "Using a mass spectrometer and the Porter method, they also detected free nonconjugated procyanidin dimers to tetramers in rat plasma after oral administration of purified oligomers to rats at a dose of 1 g/kg (Shoji et al., 2006). Pentamers were detected using the Porter method only (Shoji et al., 2006), but these data should be interpreted cautiously because Porter method lacks the specificity and accuracy to detect procyanidins in blood (Gu, 2012). The concentration of procyanidin B1 [epicatechin-(4b!8)- catechin] in human serum was 10.6 nmol/L 2 h after intake of 2.0 g of grape seed extract (Sano et al., 2003). "
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